Galectin-3 Polyklonaler Antikörper

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Galectin-3 Polyklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14979-1-PBS

Synonyme

35 kDa lectin, Carbohydrate-binding protein 35, CBP 35, CBP35, Gal 3

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  WB analysis of HeLa cells using 14979-1-AP (same clone as 14979-1-PBS)

  WB result of Galectin 3 antibody (14979-1-AP, 1:1000) with si-Control and si-Galectin 3 transfected HeLa cells. This data was developed using the same antibody clone with 14979-1-PBS in a different storage buffer formulation.

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  WB analysis using 14979-1-AP (same clone as 14979-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14979-1-AP (Galectin-3 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14979-1-PBS in a different storage buffer formulation.

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  WB analysis of human heart using 14979-1-AP (same clone as 14979-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 14979-1-AP (Galectin-3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14979-1-PBS in a different storage buffer formulation.

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  WB analysis of rat colon using 14979-1-AP (same clone as 14979-1-PBS)

  rat colon tissue were subjected to SDS PAGE followed by western blot with 14979-1-AP (Galectin-3 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14979-1-PBS in a different storage buffer formulation.

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  IP experiment of MCF-7 using 14979-1-AP (same clone as 14979-1-PBS)

  IP result of anti-Galectin-3 (IP:14979-1-AP, 3ug; Detection:14979-1-AP 1:500) with MCF-7 cells lysate 1600ug. This data was developed using the same antibody clone with 14979-1-PBS in a different storage buffer formulation.

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  IHC staining of human thyroid cancer using 14979-1-AP (same clone as 14979-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 14979-1-AP (Galectin-3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14979-1-PBS in a different storage buffer formulation.

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  IHC staining of human thyroid cancer using 14979-1-AP (same clone as 14979-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 14979-1-AP (Galectin-3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14979-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 14979-1-AP (same clone as 14979-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14979-1-AP (Galectin-3 antibody) at dilution of 1:8000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14979-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 14979-1-AP (same clone as 14979-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Galectin-3 antibody (14979-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 14979-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 14979-1-AP (same clone as 14979-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug Galectin-3 Polyclonal antibody (14979-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2) (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 14979-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

14979-1-PBS bindet in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA Galectin-3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Galectin-3 fusion protein Ag6891
• Vollständiger Name: lectin, galactoside-binding, soluble, 3
• Berechnetes Molekulargewicht: 26 kDa
• Beobachtetes Molekulargewicht: 31 kDa
• GenBank-Zugangsnummer: BC001120
• Gene symbol: Galectin-3
• Gene ID (NCBI): 3958
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-3, a member of the β-galactoside-binding proteins, contains one carbohydrate recognition domain (CRD) and a proline- and glycine-rich N-terminal domain through which is able to form oligomers (PMID: 14758078). Galectin-3 is widely expressed in many normal tissues and a variety of tumors. It is found intracellularly in nucleus and cytoplasm or secreted outside of cell, being present on the cell surface or in the extracellular space (PMID: 16478649). Galectin-3 is involved in various biological processes including cell growth, adhesion, differentiation, apoptosis, angiogenesis, immune response, neoplastic transformation and metastasis (PMID: 16478649; 14758078).