Galectin-3 Rekombinanter Antikörper

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• KD/KO Validated

Galectin-3 Rekombinant Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

4M10

Kat-Nr. : 82024-1-PBS

Synonyme

Gal 3, CBP35, CBP 35, Carbohydrate-binding protein 35, 4M10

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  WB analysis using 82024-1-RR (same clone as 82024-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82024-1-RR (Galectin 3 antibody) at dilution of 1:9900 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82024-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 82024-1-RR (same clone as 82024-1-PBS)

  WB result of Galectin 3 antibody (82024-1-RR; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Galectin 3 transfected HeLa cells. This data was developed using the same antibody clone with 82024-1-PBS in a different storage buffer formulation.

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  IP experiment of MCF-7 using 82024-1-RR (same clone as 82024-1-PBS)

  IP result of anti-Galectin 3 (IP:82024-1-RR, 4ug; Detection:82024-1-RR 1:10000) with MCF-7 cells lysate 1880 ug. This data was developed using the same antibody clone with 82024-1-PBS in a different storage buffer formulation.

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  IHC staining of human thyroid cancer using 82024-1-RR (same clone as 82024-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 82024-1-RR (Galectin 3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82024-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 82024-1-RR (same clone as 82024-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 82024-1-RR (Galectin 3 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82024-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 82024-1-RR (same clone as 82024-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Galectin 3 antibody (82024-1-RR, Clone: 4M10 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 82024-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82024-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82024-1-RR against Human Galectin 3 were performed. The affinity constant is 1.17 nM.

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Geprüfte Anwendungen

Produktinformation

82024-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA Galectin-3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Galectin-3 fusion protein Ag6891
• Vollständiger Name: lectin, galactoside-binding, soluble, 3
• Berechnetes Molekulargewicht: 26 kDa
• Beobachtetes Molekulargewicht: 31 kDa
• GenBank-Zugangsnummer: BC001120
• Gene symbol: Galectin-3
• Gene ID (NCBI): 3958
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-3, a member of the β-galactoside-binding proteins, contains one carbohydrate recognition domain (CRD) and a proline- and glycine-rich N-terminal domain through which is able to form oligomers (PMID: 14758078). Galectin-3 is widely expressed in many normal tissues and a variety of tumors. It is found intracellularly in nucleus and cytoplasm or secreted outside of cell, being present on the cell surface or in the extracellular space (PMID: 16478649). Galectin-3 is involved in various biological processes including cell growth, adhesion, differentiation, apoptosis, angiogenesis, immune response, neoplastic transformation and metastasis (PMID: 16478649; 14758078).