Galectin-3 Rekombinanter Antikörper

Galectin-3 Rekombinant Antikörper für IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242453H8

Kat-Nr. : 84889-3-PBS

Synonyme

Gal-3, Lgals3, 35 kDa lectin

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  Sandwich ELISA sample test of MP01641-1

  Serum of four mice was measured. The mouse Galectin-3 concentration of detected samples was determined to be 65.14 ng/mL with a range of 47.02 - 74.00 ng/mL.

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  Sandwich ELISA standard curve of MP01641-1

  Sandwich ELISA standard curve of MP01641-1, Mouse Galectin-3 Recombinant Matched Antibody Pair - PBS only. 84889-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1232. 84889-3-PBS was HRP conjugated as the detection antibody. Range: 9.77-625 pg/mL

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  IHC staining of mouse colon using 84889-3-RR (same clone as 84889-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 84889-3-RR (Galectin-3 antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84889-3-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 84889-3-RR (same clone as 84889-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 84889-3-RR (Galectin-3 antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84889-3-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 84889-3-RR (same clone as 84889-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84889-3-RR (Galectin-3 antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84889-3-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 84889-3-RR (same clone as 84889-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84889-3-RR (Galectin-3 antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84889-3-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01641-1

  Cytometric bead array standard curve of MP01641-1, MOUSE Galectin-3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84889-2-PBS. Detection antibody: 84889-3-PBS. Standard: Eg1232. Range: 0.469-60 ng/mL.

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  Affinity and Kinetic Characterization of 84889-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84889-3-RR against Mouse Galectin-3 were performed. The affinity constant is 57.2 pM.

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Geprüfte Anwendungen

Produktinformation

84889-3-PBS bindet in IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA Galectin-3 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Galectin-3 fusion protein Eg1232
• Vollständiger Name: lectin, galactose binding, soluble 3
• Berechnetes Molekulargewicht: 28 kDa
• Gene symbol: Lgals3
• Gene ID (NCBI): 16854
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-3, a member of the β-galactoside-binding proteins, contains one carbohydrate recognition domain (CRD) and a proline- and glycine-rich N-terminal domain through which is able to form oligomers (PMID: 14758078). Galectin-3 is widely expressed in many normal tissues and a variety of tumors. It is found intracellularly in nucleus and cytoplasm or secreted outside of cell, being present on the cell surface or in the extracellular space (PMID: 16478649). Galectin-3 is involved in various biological processes including cell growth, adhesion, differentiation, apoptosis, angiogenesis, immune response, neoplastic transformation and metastasis (PMID: 16478649; 14758078).