Galectin-3 Rekombinanter Antikörper

Galectin-3 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

242453H5

Kat-Nr. : 98335-3-RR

Synonyme

Gal-3, Lgals3, 242453H5, 35 kDa lectin

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  FC experiment of Balb/c mouse peritoneal macrophages using 98335-3-RR

  1x10^6 Balb/c mouse peritoneal macrophages were surface stained with 0.25 ug Anti-Mouse Galectin-3 Rabbit RecAb (98335-3-RR, Clone:242453H5) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with APC Anti-Mouse CD11b. Cells were not fixed.

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  FC experiment of Balb/c mouse peritoneal macrophages using 98335-3-RR

  1x10^6 Balb/c mouse peritoneal macrophages were surface stained with 0.25 ug Anti-Mouse Galectin-3 Rabbit RecAb (98335-3-RR, Clone:242453H5)(red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

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  Affinity and Kinetic Characterization of 98335-3-RR

  Biolayer interferometry (BLl) kinetic assays of 98335-3-RR against Mouse Galectin-3 were performed. The affinity constant is 49.5 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Balb/c peritoneale Makrophagen der Maus

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98335-3-RR bindet in FC Galectin-3 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Galectin-3 fusion protein Eg1232
• Vollständiger Name: lectin, galactose binding, soluble 3
• Berechnetes Molekulargewicht: 28 kDa
• Gene symbol: Lgals3
• Gene ID (NCBI): 16854
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-3, a member of the β-galactoside-binding proteins, contains one carbohydrate recognition domain (CRD) and a proline- and glycine-rich N-terminal domain through which is able to form oligomers (PMID: 14758078). Galectin-3 is widely expressed in many normal tissues and a variety of tumors. It is found intracellularly in nucleus and cytoplasm or secreted outside of cell, being present on the cell surface or in the extracellular space (PMID: 16478649). Galectin-3 is involved in various biological processes including cell growth, adhesion, differentiation, apoptosis, angiogenesis, immune response, neoplastic transformation and metastasis (PMID: 16478649; 14758078).