Galectin-4 Polyklonaler Antikörper

Galectin-4 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13391-1-PBS

Synonyme

---

• 

  WB analysis using 13391-1-AP (same clone as 13391-1-PBS)

  HT-29 cells were subjected to SDS PAGE followed by western blot with 13391-1-AP (Galectin-4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13391-1-PBS in a different storage buffer formulation.

• 

  WB analysis of COLO 320 using 13391-1-AP (same clone as 13391-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 13391-1-AP (Galectin-4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13391-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 13391-1-AP (same clone as 13391-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 13391-1-AP (Galectin-4 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 13391-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 13391-1-AP (same clone as 13391-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 13391-1-AP (Galectin-4 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 13391-1-PBS in a different storage buffer formulation.

• 

  IF Staining of COLO 320 using 13391-1-AP (same clone as 13391-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed COLO 320 cells using Galectin-4 antibody (13391-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13391-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

13391-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA Galectin-4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Galectin-4 fusion protein Ag4049
• Vollständiger Name: lectin, galactoside-binding, soluble, 4
• Berechnetes Molekulargewicht: 323 aa, 36 kDa
• Beobachtetes Molekulargewicht: 36 kDa
• GenBank-Zugangsnummer: BC034750
• Gene symbol: Galectin-4
• Gene ID (NCBI): 3960
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-4 contains two arbohydrate recognition domains (CRDs) and is expressed in the epithelium of oral mucosa, esophagus, and intestinal and colonic mucosa (PMID: 15546874; 15115909). Aberrant expression of galectin-4 has been reported in several cancers.