Galectin-4 Monoklonaler Antikörper

Galectin-4 Monoklonal Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3C2G3

Kat-Nr. : 66686-1-PBS

Synonyme

Antigen NY CO 27, Gal 4, GAL4, Galectin-4, L 36 lactose binding protein, L36LBP, Lactose binding lectin 4, LGALS4

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  WB analysis of HT-29 using 66686-1-Ig (same clone as 66686-1-PBS)

  HT-29 cells were subjected to SDS PAGE followed by western blot with 66686-1-Ig (LGALS4,GAL4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66686-1-PBS in a different storage buffer formulation.

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  WB analysis of human ileum using 66686-1-Ig (same clone as 66686-1-PBS)

  human ileum tissue were subjected to SDS PAGE followed by western blot with 66686-1-Ig (LGALS4,GAL4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66686-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 66686-1-Ig (same clone as 66686-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66686-1-Ig (Galectin-4 antibody) at dilution of 1:5000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66686-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 66686-1-Ig (same clone as 66686-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66686-1-Ig (Galectin-4 antibody) at dilution of 1:5000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66686-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 66686-1-Ig (same clone as 66686-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66686-1-Ig (Galectin-4 antibody) at dilution of 1:5000 (under 4x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66686-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 66686-1-Ig (same clone as 66686-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66686-1-Ig (LGALS4,GAL4 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66686-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 66686-1-Ig (same clone as 66686-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66686-1-Ig (LGALS4,GAL4 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66686-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66686-1-Ig (same clone as 66686-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66686-1-Ig (LGALS4,GAL4 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66686-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66686-1-Ig (same clone as 66686-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66686-1-Ig (LGALS4,GAL4 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66686-1-PBS in a different storage buffer formulation.

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  IF Staining of human colon cancer using 66686-1-Ig (same clone as 66686-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Galectin-4 antibody (66686-1-Ig, Clone: 3C2G3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66686-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human colon cancer using 66686-1-Ig (same clone as 66686-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Galectin-4 antibody (66686-1-Ig, Clone: 3C2G3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66686-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66686-1-PBS bindet in WB, IHC, IF-P, Indirect ELISA Galectin-4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Galectin-4 fusion protein Ag4049
• Vollständiger Name: lectin, galactoside-binding, soluble, 4
• Berechnetes Molekulargewicht: 323 aa, 36 kDa
• Beobachtetes Molekulargewicht: 36 kDa
• GenBank-Zugangsnummer: BC034750
• Gene symbol: Galectin-4
• Gene ID (NCBI): 3960
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-4 contains two arbohydrate recognition domains (CRDs) and is expressed in the epithelium of oral mucosa, esophagus, and intestinal and colonic mucosa (PMID: 15546874; 15115909). Aberrant expression of galectin-4 has been reported in several cancers.