Galectin-7 Polyklonaler Antikörper

Galectin-7 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 16730-1-AP

Synonyme

LGALS7B, Gal 7, GAL7, Gal-7, Galectin 7

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  WB analysis of mouse skin using 16730-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 16730-1-AP (Galectin-7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse small intestine using 16730-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 16730-1-AP (Galectin-7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human cervix using 16730-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervix using 16730-1-AP (Galectin-7 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human cervix using 16730-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervix using 16730-1-AP (Galectin-7 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of mouse skin using 16730-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 16730-1-AP (Galectin-7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skin using 16730-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 16730-1-AP (Galectin-7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushautgewebe, Maus-Dünndarmgewebe
• Erfolgreiche Detektion in IHC: humanes Zervixgewebe, Maushautgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

16730-1-AP bindet in WB, IHC, ELISA Galectin-7 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Galectin-7 fusion protein Ag10188
• Vollständiger Name: lectin, galactoside-binding, soluble, 7B
• Berechnetes Molekulargewicht: 136 aa, 15 kDa
• Beobachtetes Molekulargewicht: 14-15 kDa
• GenBank-Zugangsnummer: BC073743
• Gene symbol: Galectin-7
• Gene ID (NCBI): 653499
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-7 contains one carbohydrate recognition domain (CRD) and is biologically active as monomer. It is mainly expressed in stratified epithelium, especially in epidermis. Alterations of galectin-7 expression during cancer progression have been reported. Galectin-7 is involved in epithelial differentiation and development and may have a role in tumour development (PMID: 16429325).

Publikationen

Species	Application	Title
		EJNMMI Res; Novel GAL7-targeted fluorescent molecular imaging probe for high-grade squamous intraepithelial lesion and cervical cancer screening; Authors - Xiaohui Teng