Gamma Catenin Polyklonaler Antikörper

Gamma Catenin Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11146-1-PBS

Synonyme

JUP, JUP/gamma Catenin, γ-Catenin, ARVD12, Catenin gamma

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  WB analysis of mouse heart using 11146-1-AP (same clone as 11146-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 11146-1-AP (Plakoglobin/gamma catenin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11146-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 11146-1-AP (same clone as 11146-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 11146-1-AP (Plakoglobin/gamma catenin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11146-1-PBS in a different storage buffer formulation.

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  WB analysis of T-47D using 11146-1-AP (same clone as 11146-1-PBS)

  T-47D cells were subjected to SDS PAGE followed by western blot with 11146-1-AP (Plakoglobin/gamma catenin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11146-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 11146-1-AP (same clone as 11146-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 11146-1-AP (Plakoglobin/gamma catenin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11146-1-PBS in a different storage buffer formulation.

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  WB analysis of human heart using 11146-1-AP (same clone as 11146-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 11146-1-AP (Plakoglobin/gamma catenin antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11146-1-PBS in a different storage buffer formulation.

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  IP experiment of HT-29 using 11146-1-AP (same clone as 11146-1-PBS)

  IP result of anti-Gamma Catenin (IP:11146-1-AP, 4ug; Detection:11146-1-AP 1:1000) with HT-29 cells lysate 1040 ug. This data was developed using the same antibody clone with 11146-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 11146-1-AP (same clone as 11146-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11146-1-AP (Plakoglobin/gamma catenin antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11146-1-PBS in a different storage buffer formulation.

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  IHC staining of human bowen disease using 11146-1-AP (same clone as 11146-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 11146-1-AP (Gamma Catenin antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11146-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human bowen disease using 11146-1-AP (same clone as 11146-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 11146-1-AP (Gamma Catenin antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11146-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human bowen disease using 11146-1-AP (same clone as 11146-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 11146-1-AP (Gamma Catenin antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11146-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 11146-1-AP (same clone as 11146-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 11146-1-AP (Plakoglobin/gamma catenin antibody at dilution of 1:50. This data was developed using the same antibody clone with 11146-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 11146-1-AP (same clone as 11146-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 11146-1-AP (Plakoglobin/gamma catenin antibody at dilution of 1:50. This data was developed using the same antibody clone with 11146-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin using 11146-1-AP (same clone as 11146-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin slide using 11146-1-AP (Plakoglobin/gamma catenin antibody at dilution of 1:50. This data was developed using the same antibody clone with 11146-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin using 11146-1-AP (same clone as 11146-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin slide using 11146-1-AP (Plakoglobin/gamma catenin antibody at dilution of 1:50. This data was developed using the same antibody clone with 11146-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 11146-1-AP (same clone as 11146-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 11146-1-AP (Plakoglobin/gamma catenin antibody at dilution of 1:50. This data was developed using the same antibody clone with 11146-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 11146-1-AP (same clone as 11146-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 11146-1-AP (Plakoglobin/gamma catenin antibody at dilution of 1:50. This data was developed using the same antibody clone with 11146-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

11146-1-PBS bindet in WB, IHC, IP, Indirect ELISA Gamma Catenin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Gamma Catenin fusion protein Ag1627
• Vollständiger Name: junction plakoglobin
• Berechnetes Molekulargewicht: 82 kDa
• Beobachtetes Molekulargewicht: 82 kDa
• GenBank-Zugangsnummer: BC011865
• Gene symbol: Gamma Catenin
• Gene ID (NCBI): 3728
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Plakoglobin (γ-catenin), a member of the Armadillo proteins, plays important roles in cell adhesion with α-catenin and β-catenin, is involved in Wnt signaling, and is translocated into the nucleus and binds LEF1. Plakoglobin is a major cytoplasmic component of both desmosomes and adherens junctions, and is the only known constituent common to submembranous plaques in both of these structures, which are located at the intercalated disc (ICD) of cardiomyocytes. Plakoglobin links cadherins to the actin cytoskeleton. Mutations in plakoglobin are associated with arrhythmogenic right ventricular dysplasia and Pemphigus vulgaris.