Gamma Catenin Monoklonaler Antikörper

Gamma Catenin Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Hund, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1A5D10

Kat-Nr. : 66445-1-PBS

Synonyme

JUP, JUP/gamma Catenin, γ-Catenin, ARVD12, Catenin gamma

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  WB analysis of human heart using 66445-1-Ig (same clone as 66445-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 66445-1-Ig (Plakoglobin/gamma catenin antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66445-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat heart using 66445-1-Ig (same clone as 66445-1-PBS)

  rat heart tissue were subjected to SDS PAGE followed by western blot with 66445-1-Ig (Plakoglobin/gamma catenin antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66445-1-PBS in a different storage buffer formulation.

• 

  WB analysis of T-47D using 66445-1-Ig (same clone as 66445-1-PBS)

  T-47D cells were subjected to SDS PAGE followed by western blot with 66445-1-Ig (gamma catenin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66445-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A431 using 66445-1-Ig (same clone as 66445-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 66445-1-Ig (gamma catenin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66445-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human heart using 66445-1-Ig (same clone as 66445-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 66445-1-Ig (gamma catenin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66445-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat heart using 66445-1-Ig (same clone as 66445-1-PBS)

  rat heart tissue were subjected to SDS PAGE followed by western blot with 66445-1-Ig (gamma catenin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66445-1-PBS in a different storage buffer formulation.

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  WB analysis of MDCK using 66445-1-Ig (same clone as 66445-1-PBS)

  MDCK cells were subjected to SDS PAGE followed by western blot with 66445-1-Ig (gamma catenin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66445-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HT-29 using 66445-1-Ig (same clone as 66445-1-PBS)

  HT-29 cells were subjected to SDS PAGE followed by western blot with 66445-1-Ig (gamma catenin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66445-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66445-1-Ig (same clone as 66445-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66445-1-Ig (gamma catenin antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66445-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66445-1-Ig (same clone as 66445-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66445-1-Ig (gamma catenin antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66445-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 66445-1-Ig (same clone as 66445-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66445-1-Ig (Gamma Catenin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66445-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 66445-1-Ig (same clone as 66445-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66445-1-Ig (Gamma Catenin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66445-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat stomach using 66445-1-Ig (same clone as 66445-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 66445-1-Ig (Gamma Catenin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66445-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat stomach using 66445-1-Ig (same clone as 66445-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 66445-1-Ig (Gamma Catenin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66445-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse stomach using 66445-1-Ig (same clone as 66445-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 66445-1-Ig (Gamma Catenin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66445-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse stomach using 66445-1-Ig (same clone as 66445-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 66445-1-Ig (Gamma Catenin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66445-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin using 66445-1-Ig (same clone as 66445-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 66445-1-Ig (Plakoglobin/gamma catenin antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66445-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin using 66445-1-Ig (same clone as 66445-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 66445-1-Ig (Plakoglobin/gamma catenin antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66445-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 66445-1-Ig (same clone as 66445-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using Gamma Catenin antibody (66445-1-Ig, Clone: 1A5D10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66445-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 66445-1-Ig (same clone as 66445-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using Gamma Catenin antibody (66445-1-Ig, Clone: 1A5D10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66445-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66445-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA Gamma Catenin und zeigt Reaktivität mit Hausschwein, human, Hund, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Hund, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Gamma Catenin fusion protein Ag1627
• Vollständiger Name: junction plakoglobin
• Berechnetes Molekulargewicht: 82 kDa
• Beobachtetes Molekulargewicht: 82 kDa
• GenBank-Zugangsnummer: BC011865
• Gene symbol: Gamma Catenin
• Gene ID (NCBI): 3728
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Plakoglobin (γ-catenin), a member of the Armadillo proteins, plays important roles in cell adhesion with α-catenin and β-catenin, is involved in Wnt signaling, and is translocated into the nucleus and binds LEF1. Plakoglobin is a major cytoplasmic component of both desmosomes and adherens junctions, and is the only known constituent common to submembranous plaques in both of these structures, which are located at the intercalated disc (ICD) of cardiomyocytes. Plakoglobin links cadherins to the actin cytoskeleton. Mutations in plakoglobin are associated with arrhythmogenic right ventricular dysplasia and Pemphigus vulgaris.