Gamma Tubulin Monoklonaler Antikörper

Gamma Tubulin Monoklonal Antikörper für WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Hund, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3F9H8

Kat-Nr. : 66320-1-PBS

Synonyme

TUBG1, TUBG, GCP-1, Gamma-tubulin complex component 1, Gamma-1-tubulin

---

• 

  WB analysis using 66320-1-Ig (same clone as 66320-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66320-1-Ig (gamma tubulin antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66320-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 66320-1-Ig (same clone as 66320-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66320-1-Ig (gamma tubulin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66320-1-PBS in a different storage buffer formulation.

• 

  WB analysis of EC109 using 66320-1-Ig (same clone as 66320-1-PBS)

  EC109 cells were subjected to SDS PAGE followed by western blot with 66320-1-Ig (gamma tubulin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66320-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 66320-1-Ig (same clone as 66320-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 66320-1-Ig (gamma tubulin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66320-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66320-1-Ig (same clone as 66320-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66320-1-Ig (gamma tubulin antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66320-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66320-1-Ig (same clone as 66320-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66320-1-Ig (gamma tubulin antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66320-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 66320-1-Ig (same clone as 66320-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66320-1-Ig (gamma tubulin antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66320-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 66320-1-Ig (same clone as 66320-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66320-1-Ig (gamma tubulin antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66320-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MDCK using 66320-1-Ig (same clone as 66320-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed MDCK cells using 66320-1-Ig (gamma tubulin antibody) at dilution of 1:200 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66320-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MDCK using 66320-1-Ig (same clone as 66320-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed MDCK cells using 66320-1-Ig (gamma tubulin antibody) at dilution of 1:200 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66320-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A549 using 66320-1-Ig (same clone as 66320-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using Gamma Tubulin antibody (66320-1-Ig, Clone: 3F9H8 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), Alpha Tubulin antibody (11224-1-AP, red). This data was developed using the same antibody clone with 66320-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 66320-1-Ig (same clone as 66320-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 66320-1-Ig (gamma tubulin antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66320-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 66320-1-Ig (same clone as 66320-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Gamma Tubulin (66320-1-Ig, Clone:3F9H8) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66320-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

66320-1-PBS bindet in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Gamma Tubulin und zeigt Reaktivität mit human, Hund, Maus, Ratten

• Getestete Reaktivität: human, Hund, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Gamma Tubulin fusion protein Ag23913
• Vollständiger Name: tubulin, gamma 1
• Berechnetes Molekulargewicht: 51 kDa
• Beobachtetes Molekulargewicht: 48-55 kDa
• GenBank-Zugangsnummer: BC000619
• Gene symbol: Gamma Tubulin
• Gene ID (NCBI): 7283
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Gamma tubulin is a member of tubulin superfamily and is a key component required for microtubule nucleation and stabilization. It is concentrated at the pericentriolar material of centrosomes in interphase cells, predominantly on spindle poles in mitotic cells, while found in midbodies during cytokinesis. Overexpression of gamma tubulin has been found in various cancers including breast cancer and gliomas. This antibody can well label the centrosome structure.