Gelsolin Monoklonaler Antikörper

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• KD/KO Validated

Gelsolin Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgA

Getestete Reaktivität

human

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3C4A2

Kat-Nr. : 66280-1-PBS

Synonyme

3C4A2, Actin-depolymerizing factor, GSN

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  WB analysis of U2OS using 66280-1-Ig (same clone as 66280-1-PBS)

  U2OS (WT and GSN KO) lysates prepared with RIPA buffer, 25 μg protein loaded. 66280-1-Ig incubated at 1:1000 at 4°C overnight in 5% BSA in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency. This data was developed using the same antibody clone with 66280-1-PBS in a different storage buffer formulation.

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  WB analysis of human plasma using 66280-1-Ig (same clone as 66280-1-PBS)

  human plasma were subjected to SDS PAGE followed by western blot with 66280-1-Ig (Gelsolin Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66280-1-PBS in a different storage buffer formulation.

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  WB analysis of HT-29 using 66280-1-Ig (same clone as 66280-1-PBS)

  HT-29 cells were subjected to SDS PAGE followed by western blot with 66280-1-Ig (Gelsolin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66280-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 66280-1-Ig (same clone as 66280-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 66280-1-Ig (Gelsolin Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66280-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 66280-1-Ig (same clone as 66280-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66280-1-Ig (Gelsolin Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66280-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 66280-1-Ig (same clone as 66280-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66280-1-Ig (Gelsolin Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66280-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66280-1-Ig (same clone as 66280-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66280-1-Ig (Gelsolin Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66280-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66280-1-Ig (same clone as 66280-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66280-1-Ig (Gelsolin Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66280-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66280-1-PBS bindet in WB, IHC, Indirect ELISA Gelsolin und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgA
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Gelsolin fusion protein Ag17916
• Vollständiger Name: gelsolin (amyloidosis, Finnish type)
• Berechnetes Molekulargewicht: 782 aa, 86 kDa
• Beobachtetes Molekulargewicht: 80-83 kDa
• GenBank-Zugangsnummer: BC026033
• Gene symbol: Gelsolin
• Gene ID (NCBI): 2934
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Thiophiler Affinitätschromatograph
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Gelsolin (GSN) is an actin binding protein that regulates actin-severing/depolymerizing in a calcium dependent manner, and acts as a multifunctional regulator for cell metabolism and survival. Gelsolin exists in two isoforms: a cytoplasmic gelsolin (cGSN; 80-90 kDa) and a plasma gelsolin (pGSN; 93 kDa), differing at N terminal residues. Decreased levels of pGSN are strongly associated with human diseases, such as, acute liver injury, major trauma, myocardial infarction, inflammation, lung injury, rheumatoid arthritis, hemodialysis, multiple sclerosis, Alzheimer's disease.