Geminin Rekombinanter Antikörper

Geminin Rekombinant Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240835B5

Kat-Nr. : 84199-2-PBS

Synonyme

GMNN, 240835B5, Gem, MGORS6

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  WB analysis of Jurkat using 84199-2-RR (same clone as 84199-2-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 84199-2-RR (GMNN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84199-2-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 84199-2-RR (same clone as 84199-2-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 84199-2-RR (GMNN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84199-2-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 84199-2-RR (same clone as 84199-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84199-2-RR (Geminin antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84199-2-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 84199-2-RR (same clone as 84199-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Geminin antibody (84199-2-RR, Clone: 240835B5 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84199-2-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 84199-2-RR (same clone as 84199-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Geminin antibody (84199-2-RR, Clone: 240835B5 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84199-2-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 84199-2-RR (same clone as 84199-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Geminin antibody (84199-2-RR, Clone: 240835B5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84199-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84199-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84199-2-RR against Human Geminin were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

84199-2-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA Geminin und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Geminin fusion protein Ag1076
• Vollständiger Name: geminin, DNA replication inhibitor
• Berechnetes Molekulargewicht: 24 kDa
• Beobachtetes Molekulargewicht: 28-29 kDa
• GenBank-Zugangsnummer: BC005185
• Gene symbol: GMNN
• Gene ID (NCBI): 51053
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GMNN, also designated geminin, contains 212 amino acids and has a destruction box sequence (RRTLKVIQP). GMNN participates in inhibiting DNA replication by preventing the incorporation of MCM complex into the pre-replication complex (pre-RC). It is degraded during the metaphase-anaphase transition of cell cycle's mitotic phase, which permits replication in the succeeding cell cycle. GMNN has a broad sedimentation profile ranging from about 25 kDa to 90 kDa, with a major peak at 30 kDa. Scanning of the signals shows that discrete peaks corresponding to the apparent mass of 42.5 kDa and 66 kDa are present. The dimer of geminin(50 kDa) can also be detected. (PMID: 15313623)