Glucocorticoid receptor Polyklonaler Antikörper

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• KD/KO Validated

Glucocorticoid receptor Polyklonal Antikörper für IF/ICC, FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF/ICC, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Publikationen(1)

Kat-Nr. : CL488-24050

Synonyme

NR3C1, GRL, GR, GCR, GCCR

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  IF Staining of A549 using CL488-24050

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using CoraLite® Plus 488 Glucocorticoid receptor antibody (CL488-24050) at dilution of 1:200.

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  FC experiment of HeLa using CL488-24050

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Glucocorticoid receptor (CL488-24050) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: A549-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

CL488-24050 bindet in IF/ICC, FC (Intra) Glucocorticoid receptor und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Glucocorticoid receptor fusion protein Ag21146
• Vollständiger Name: nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)
• Berechnetes Molekulargewicht: 86 kDa
• Beobachtetes Molekulargewicht: 94-97 kDa
• GenBank-Zugangsnummer: BC015610
• Gene symbol: Glucocorticoid receptor
• Gene ID (NCBI): 2908
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

NR3C1 is a receptor for glucocorticoids, which owns a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE) and as a modulator of other transcription factors. It is involved in cell proliferation and differentiation and specifically implicated in newborn birth weight, thus providing a biological mechanism by which NR3C1 expression may influence birth weight [PMID:22810058].

Publikationen

Species	Application	Title
mouse	IF	Curr Biol; Hypothalamic AgRP neurons exert top-down control on systemic TNF-α release during endotoxemia; Authors - Mehdi Boutagouga Boudjadja