Glutamate receptor 2 Polyklonaler Antikörper

Glutamate receptor 2 Polyklonal Antikörper für WB, IHC, IF-P, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11994-1-PBS

Synonyme

GRIA2, GLUR2, GluR K2, GluR B, GluR 2

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  WB analysis using 11994-1-AP (same clone as 11994-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11994-1-AP (Glutamate receptor 2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11994-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 11994-1-AP (same clone as 11994-1-PBS)

  IP result of anti-Glutamate receptor 2 (IP:11994-1-AP, 4ug; Detection:11994-1-AP 1:2000) with mouse brain tissue lysate 2100 ug. This data was developed using the same antibody clone with 11994-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 11994-1-AP (same clone as 11994-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11994-1-AP (Glutamate receptor 2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11994-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse eye using 11994-1-AP (same clone as 11994-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using Glutamate receptor 2 antibody (11994-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11994-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse eye using 11994-1-AP (same clone as 11994-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using Glutamate receptor 2 antibody (11994-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11994-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

11994-1-PBS bindet in WB, IHC, IF-P, IP, Indirect ELISA Glutamate receptor 2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Glutamate receptor 2 fusion protein Ag2662
• Vollständiger Name: glutamate receptor, ionotropic, AMPA 2
• Berechnetes Molekulargewicht: 883 aa, 99 kDa
• Beobachtetes Molekulargewicht: 94-105 kDa
• GenBank-Zugangsnummer: BC010574
• Gene symbol: GRIA2
• Gene ID (NCBI): 2891
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. GRIA2 (glutamate receptor 2, GluR-2) belongs to a family of glutamate receptors that are sensitive to alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA), and function as ligand-activated cation channels that mediate the fast component of excitatory postsynaptic currents in neurons of the central nervous system. These channels are assembled from 4 related subunits, GRIA1-4. The presence of GRIA2 in the heteromeric AMPA receptors impermeabilizes it to calcium, preventing possible calcium-mediated toxicity.