Glutamine Synthetase Polyklonaler Antikörper

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• KD/KO Validated

Glutamine Synthetase Polyklonal Antikörper für WB, IHC, IF-P, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11037-2-PBS

Synonyme

GLUL, EC:2.3.1.225, EC:6.3.1.2, GLNS, Glutamate--ammonia ligase

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  WB analysis of Jurkat using 11037-2-AP (same clone as 11037-2-PBS)

  WB result of Glutamine synthetase antibody (11037-2-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Glutamine synthetase transfected Jurkat cells. This data was developed using the same antibody clone with 11037-2-PBS in a different storage buffer formulation.

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  WB analysis using 11037-2-AP (same clone as 11037-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11037-2-AP (Glutamine Synthetase antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11037-2-PBS in a different storage buffer formulation.

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  IP experiment of mouse liver using 11037-2-AP (same clone as 11037-2-PBS)

  IP result of anti-Glutamine Synthetase (IP:11037-2-AP, 4ug; Detection:11037-2-AP 1:5000) with mouse liver tissue lysate 2400 ug. This data was developed using the same antibody clone with 11037-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 11037-2-AP (same clone as 11037-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11037-2-AP (Glutamine Synthetase antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11037-2-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 11037-2-AP (same clone as 11037-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11037-2-AP (Glutamine Synthetase antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11037-2-PBS in a different storage buffer formulation.

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  IF Staining of mouse liver using 11037-2-AP (same clone as 11037-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using Glutamine Synthetase antibody (11037-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11037-2-PBS in a different storage buffer formulation.

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  IF Staining of mouse eye using 11037-2-AP (same clone as 11037-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using Glutamine Synthetase antibody (11037-2-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11037-2-PBS in a different storage buffer formulation.

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  IF Staining of human brain using 11037-2-AP (same clone as 11037-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human brain tissue using 11037-2-AP (Glutamine synthetase antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11037-2-PBS in a different storage buffer formulation.

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  FC experiment of Jurkat using 11037-2-AP (same clone as 11037-2-PBS)

  1x10^6 Jurkat cells were intracellularly stained with 0.4 ug Glutamine Synthetase Polyclonal antibody (11037-2-AP)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 11037-2-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

11037-2-PBS bindet in WB, IHC, IF-P, FC (Intra), IP, ELISA Glutamine Synthetase und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Glutamine Synthetase fusion protein Ag1510
• Vollständiger Name: glutamate-ammonia ligase (glutamine synthetase)
• Berechnetes Molekulargewicht: 374 aa, 42 kDa
• Beobachtetes Molekulargewicht: 40-42 kDa
• GenBank-Zugangsnummer: BC011700
• Gene symbol: Glutamine Synthetase
• Gene ID (NCBI): 2752
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GLUL(Glutamine synthetase) is also named as GS,GLNS and belongs to the glutamine synthetase family.This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner By similarity. Essential for proliferation of fetal skin fibroblasts(PMID:18662667).Defects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD).Organismal glutamine production is augmented secondary to an increase in the activity of glutamine synthetase in the lung and skeletal muscle(PMID:7630137). There are other bands with higher (66 kDa, 97 kDa) and lower (30 kDa)molecular weights also detected besides the 42 kDa band indicating the proteolysis of GLUL protein by the ubiquitin system(PMID:10091759).