Glutamine synthetase Rekombinanter Antikörper

Glutamine synthetase Rekombinant Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

CloneNo.

251711A8

Kat-Nr. : 86716-1-RR

Synonyme

GLUL, EC:2.3.1.225, EC:6.3.1.2, GLNS, Glutamate--ammonia ligase

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  WB analysis using 86716-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86716-1-RR (Glutamine synthetase antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 86716-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86716-1-RR (Glutamine synthetase antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 86716-1-RR

  IP result of anti-Glutamine synthetase (IP:86716-1-RR, 4ug; Detection:86716-1-RR 1:2000) with mouse liver tissue lysate 2480 ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, HEK-293-Zellen, HeLa-Zellen, Maushirngewebe, Rattenlebergewebe
• Erfolgreiche IP: Mauslebergewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86716-1-RR bindet in WB, IP, ELISA Glutamine synthetase und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Glutamine synthetase fusion protein Ag1510
• Vollständiger Name: glutamate-ammonia ligase (glutamine synthetase)
• Berechnetes Molekulargewicht: 374 aa, 42 kDa
• Beobachtetes Molekulargewicht: 40-42 kDa
• GenBank-Zugangsnummer: BC011700
• Gene symbol: Glutamine Synthetase
• Gene ID (NCBI): 2752
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GLUL(Glutamine synthetase) is also named as GS,GLNS and belongs to the glutamine synthetase family. This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner By similarity. Essential for proliferation of fetal skin fibroblasts(PMID:18662667).Defects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD).Organismal glutamine production is augmented secondary to an increase in the activity of glutamine synthetase in the lung and skeletal muscle(PMID:7630137).