GM-CSF Rekombinanter Antikörper

GM-CSF Rekombinant Antikörper für Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

230286G9

Kat-Nr. : 82861-10-PBS

Synonyme

Csf2, Colony Stimulating Factor 2, CSF 2, Csfgm, Gm CSf

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  Sandwich ELISA standard curve of MP00306-3

  Sandwich ELISA standard curve of MP00306-3, GM-CSF Recombinant Matched Antibody Pair - PBS only. 82861-10-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0512.82861-8-PBS was HRP conjugated as the detection antibody. Range: 3.13-200 pg/mL.

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  Sample test of MP00306-3

  Spleen and lung from one mouse were chopped into 1-2 mm pieces and seeded into approximately 30 mL of media containing RPMI, 10% fetal bovine serum, 50 μM β-mercaptoethanol, and L-glutamine. The mean GM-CSF concentration was determined to be 9.5 pg/mL in spleen supernatant, 500.5 pg/mL in lung supernatant.

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  Affinity and Kinetic Characterization of 82861-10-PBS

  Biolayer interferometry (BLl) kinetic assay of 82861-10-PBS against Mouse GM-CSF was performed. The affinity constant is 4.38 nM.

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Geprüfte Anwendungen

Produktinformation

82861-10-PBS bindet in Sandwich ELISA, Indirect ELISA, Sample test GM-CSF und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: GM-CSF fusion protein Eg0512
• Vollständiger Name: colony stimulating factor 2 (granulocyte-macrophage)
• GenBank-Zugangsnummer: NM-009969
• Gene symbol: GM-CSF
• Gene ID (NCBI): 12981
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.