GM-CSF Rekombinanter Antikörper

GM-CSF Rekombinant Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC (Intra)

Konjugation

Unkonjugiert

CloneNo.

230286E11

Kat-Nr. : 98014-1-PBS

Synonyme

Csf2, Gm CSf, Csfgm, CSF 2, Colony Stimulating Factor 2

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  FC experiment of C57BL/6 CD3 T cells using 98014-1-RR (same clone as 98014-1-PBS)

  1x10^6 unstimulated or PMA and ionomycin in presence of protein transport inhibitors stimulated C57BL/6 CD3 T cells were intracellularly stained with 0.25 ug Anti-Mouse Gm-csf (98014-1-RR, Clone: 230286E11) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cell were then stained with FITC Plus Anti-Mouse CD3. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98014-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98014-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98014-1-RR against Human Gm-csf were performed. The affinity constant is 2.25 nM.

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Geprüfte Anwendungen

Produktinformation

98014-1-PBS bindet in FC (Intra) GM-CSF und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: colony stimulating factor 2 (granulocyte-macrophage)
• GenBank-Zugangsnummer: NM-009969
• Gene symbol: GM-CSF
• Gene ID (NCBI): 12981
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C.

Hintergrundinformationen

Gm-csf, also known as Csf2, is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, NK cells, endothelial cells and fibroblasts that functions as a cytokine. Gm-csf was first characterized as a hematopoietic growth factor that stimulates the proliferation of myeloid cells from bone-marrow progenitors. Gm-csf is now recognized as an important activating and differentiation factor for immune cells, and is essential for a wide range of biological processes in both innate and adaptive immunity. Gm-csf has been shown to protect against pulmonary infection and intestinal inflammation, and it is necessary for normal pulmonary and colon homeostasis.