GPNMB Rekombinanter Antikörper

GPNMB Rekombinant Antikörper für WB, Sandwich ELISA, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus, Ratte

Anwendung

WB, Sandwich ELISA, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250309C11

Kat-Nr. : 86874-1-PBS

Synonyme

Dchil, DC-HIL, Dendritic cell-associated transmembrane protein, Hgfin, Nmb

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  WB analysis of RAW 264.7 using 86874-1-RR (same clone as 86874-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 86874-1-RR (Gpnmb antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86874-1-PBS in a different storage buffer formulation.

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  WB analysis using 86874-1-RR (same clone as 86874-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86874-1-RR (Gpnmb antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86874-1-PBS in a different storage buffer formulation.

• 

  Sandwich ELISA sample test of MP02737-1

  Serum of six mice was measured. The GPNMB concentration of detected samples was determined to be 14.00 ng/mL with a range of 10.09-19.47 ng/mL.

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  Sandwich ELISA standard curve of MP02737-1

  Sandwich ELISA standard curve of MP02737-1, Mouse GPNMB Recombinant Matched Antibody Pair - PBS only. 86874-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg2814. 86874-2-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL.

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Geprüfte Anwendungen

Produktinformation

86874-1-PBS bindet in WB, Sandwich ELISA, Indirect ELISA GPNMB und zeigt Reaktivität mit Maus, Ratten

• Getestete Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: GPNMB fusion protein Eg2814
• Vollständiger Name: glycoprotein (transmembrane) nmb
• Berechnetes Molekulargewicht: 64 kDa
• Beobachtetes Molekulargewicht: 90-100 kDa, 120kDa
• GenBank-Zugangsnummer: NM_053110.4
• Gene symbol: Gpnmb
• Gene ID (NCBI): 93695
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GPNMB also known as HGFIN, osteoactivin, and DC-HIL, is a type I membrane glycoprotein involved in various biological processes, including inflammation, invasion and metastasis of malignant tumors, cell differentiation, and tissue regeneration. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB acts as an osteogenic factor that stimulates osteoblast differentiation in vivo and in vitro.