GPNMB Rekombinanter Antikörper

GPNMB Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

250309C11

Kat-Nr. : 86874-1-RR

Synonyme

Dchil, DC-HIL, Dendritic cell-associated transmembrane protein, Hgfin, Nmb

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  WB analysis of RAW 264.7 using 86874-1-RR

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 86874-1-RR (Gpnmb antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 86874-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86874-1-RR (Gpnmb antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: RAW 264.7-Zellen, Maushautgewebe, Rattenhautgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86874-1-RR bindet in WB, ELISA GPNMB und zeigt Reaktivität mit Maus, Ratten

• Getestete Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: GPNMB fusion protein Eg2814
• Vollständiger Name: glycoprotein (transmembrane) nmb
• Berechnetes Molekulargewicht: 64 kDa
• Beobachtetes Molekulargewicht: 90-100 kDa, 120kDa
• GenBank-Zugangsnummer: NM_053110.4
• Gene symbol: Gpnmb
• Gene ID (NCBI): 93695
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GPNMB also known as HGFIN, osteoactivin, and DC-HIL, is a type I membrane glycoprotein involved in various biological processes, including inflammation, invasion and metastasis of malignant tumors, cell differentiation, and tissue regeneration. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB acts as an osteogenic factor that stimulates osteoblast differentiation in vivo and in vitro.