Progranulin/PGRN Polyklonaler Antikörper

Progranulin/PGRN Polyklonal Antikörper für IHC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IHC

Konjugation

Biotin

Kat-Nr. : Biotin-18410

Synonyme

GEP, GP88, Granulin, GRN, Progranulin

---

Galerie der Validierungsdaten

• 

  IHC staining of human kidney using Biotin-18410

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using Biotin-18410(Granulin antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using Biotin-18410

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using Biotin-18410(Granulin antibody) at dilution of 1:200 (under 40x lens).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

Biotin-18410 bindet in IHC Progranulin/PGRN und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Progranulin/PGRN fusion protein Ag13178
• Vollständiger Name: granulin
• Berechnetes Molekulargewicht: 64 kDa
• GenBank-Zugangsnummer: BC000324
• Gene symbol: GRN
• Gene ID (NCBI): 2896
• Konjugation: Biotin
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 50% Glyzerin, 0,03% Proclin300, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GRN, also known as PGRN or PCDGF, is a cysteine-rich protein of 68.5 kDa that is typically secreted into a highly glycosylated 88 kDa form. PGRN is a unique growth factor that plays an important role in cutaneous wound healing. It has an anti-inflammatory effect and promotes cell proliferation. When PCDGF is degraded to several 6-25 kDa fragments, called granulins (GRNs) by neutrophil proteases, a pro-inflammatory reaction occurs. PGRN is widely expressed, particularly in epithelial cells, immune cells, neurons, and chondrocytes. High levels of PGRN expression have been reported in human cancers, and its expression is closely correlated with the development and metastasis of several cancers. The recent discovery that mutations in the gene encoding for pro-granulin (GRN) cause frontotemporal lobar degeneration (FTLD), and other neurodegenerative diseases leading to dementia, has brought renewed interest in progranulin and its functions in the central nervous system. This antibody is conjugated with Biotin.