Granzyme A Rekombinanter Antikörper

Granzyme A Rekombinant Antikörper für IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241979B8

Kat-Nr. : 84583-3-PBS

Synonyme

GrA, GZMA, Fragmentin 1, EC:3.4.21.78, Cytotoxic T-lymphocyte proteinase 1

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  IF Staining of Jurkat using 84583-3-RR (same clone as 84583-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using GZMA antibody (84583-3-RR, Clone: 241979B8 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84583-3-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

84583-3-PBS bindet in IF/ICC, Indirect ELISA Granzyme A und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Granzyme A fusion protein Ag1818
• Vollständiger Name: granzyme A (granzyme 1, cytotoxic T-lymphocyte-associated serine esterase 3)
• Berechnetes Molekulargewicht: 29 kDa
• GenBank-Zugangsnummer: BC015739
• Gene symbol: Granzyme A
• Gene ID (NCBI): 3001
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Granzyme A(GrA, a tryptase) is necessary for target cell lysis in cell-mediated immune responses. It activates mitochondrial outer membrane permeabilization- and caspase-independent cell death pathways by cleaving the mitochondrial complex I component NDUFS3after lys56. Among serine proteases GrA has an unique quaternary structure consisting of a disulphide-linked homodimer of 60kDa linked via Cys93.(PMID: 12819769) This protein has 2 isoforms produced by alternative promoter usage with the MW of 29 and 27 kDa.