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Granzyme B Rekombinanter Antikörper

Granzyme B Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

240761C6

Kat-Nr. : 83763-5-PBS

Synonyme

GZMB, CSP B, CGL1, CGL 1, CCPI

Filter:
  • Western Blot (WB) analysis of NK-92 cells using Granzyme B Recombinant antibody (83763-5-RR)

    WB analysis of NK-92 using 83763-5-RR (same clone as 83763-5-PBS)

    NK-92 cells were subjected to SDS PAGE followed by western blot with 83763-5-RR (GZMB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83763-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83763-5-RR

    Affinity and Kinetic Characterization of 83763-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83763-5-RR against Human Granzyme B were performed. The affinity constant is 0.281 nM.

Geprüfte Anwendungen

Produktinformation

83763-5-PBS bindet in WB, ELISA Granzyme B und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen Fusionsprotein
Vollständiger Name granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1)
Berechnetes Molekulargewicht 247 aa, 28 kDa
Beobachtetes Molekulargewicht 33 kDa
GenBank-ZugangsnummerBC030195
Gene symbol Granzyme B
Gene ID (NCBI) 3002
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein A purfication
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GZMB(Granzyme B) is also named as CGL1, CSPB, CTLA1, GRB and belongs to the Granzyme subfamily. This enzyme is necessary for target cell lysis in cell-mediated immune responses. The cytotoxic lymphocyte protease granzyme B (GzmB) can promote apoptosis through direct processing and activation of members of the caspase family. GzmB can also cleave the BH3-only protein, BID, to promote caspase-independent mitochondrial permeabilization (PMID:17283187). GzmB induces laminB degradation in isolated nuclei less efficiently than GzmA (PMID:11331782). This full length protein has 2 glycosylation sites and a signal peptide. Unglycosylated human granzyme B is 26 kDa and high mannose glycosylated is 32 kDa and only 32kDa or smaller forms of granzyme B are accumulated within nuclei (PMID:8626751). GzmB also forms dimers.