Granzyme B Rekombinanter Antikörper

Granzyme B Rekombinant Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC (Intra)

Konjugation

Unkonjugiert

CloneNo.

240761G4

Kat-Nr. : 98070-1-PBS

Synonyme

GZMB, 240761G4, Cathepsin G-like 1, CCPI, CGL 1

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  FC experiment of human PBMCs using 98070-1-RR (same clone as 98070-1-PBS)

  1x10^6 human PBMCs were intracellularly stained with 0.25 ug Anti-Human Granzyme B Rabbit Recombinant Antibody (98070-1-RR, Clone:240761G4) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), and 0.25 ug APC Anti-Human CD8a (OKT8) (APC-65135, Clone: OKT8), and 0.25 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98070-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 98070-1-RR (same clone as 98070-1-PBS)

  1x10^6 human PBMCs were intracellularly stained with 0.25 ug Anti-Human Granzyme B Rabbit Recombinant Antibody (98070-1-RR, Clone:240761G4) or isotype control and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG. Cells were stained with APC Anti-Human CD8a before fixing. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98070-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 98070-1-RR (same clone as 98070-1-PBS)

  1x10^6 human PBMCs were stained with 0.25 ug Anti-Human Granzyme B Rabbit Recombinant Antibody (98070-1-RR, Clone:240761G4) (red), or Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98070-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98070-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98070-1-RR against Human Granzyme B were performed. The affinity constant is 0.144 nM.

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Geprüfte Anwendungen

Produktinformation

98070-1-PBS bindet in FC (Intra) Granzyme B und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Granzyme B fusion protein Eg0592
• Vollständiger Name: granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1)
• Berechnetes Molekulargewicht: 247 aa, 28 kDa
• GenBank-Zugangsnummer: BC030195
• Gene symbol: Granzyme B
• Gene ID (NCBI): 3002
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C.

Hintergrundinformationen

GZMB (Granzyme B) is also named as CGL1, CSPB, CTLA1, GRB and belongs to the Granzyme subfamily. This enzyme is necessary for target cell lysis in cell-mediated immune responses. The cytotoxic lymphocyte protease granzyme B can promote apoptosis through direct processing and activation of members of the caspase family. Granzyme B can also cleave the BH3-only protein, BID, to promote caspase-independent mitochondrial permeabilization (PMID:17283187). Granzyme B induces lamin B degradation in isolated nuclei less efficiently than Granzyme A (PMID:11331782).