Granzyme K Monoklonaler Antikörper

Granzyme K Monoklonal Antikörper für IHC, IF-P, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

IHC, IF-P, ELISA

Konjugation

Unkonjugiert

CloneNo.

1C3A4

Kat-Nr. : 67272-1-PBS

Synonyme

GZMK, Granzyme3, Granzyme 3, Fragmentin-3, Fragmentin 3

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  IHC staining of human tonsillitis using 67272-1-Ig (same clone as 67272-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67272-1-Ig (GZMK antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67272-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 67272-1-Ig (same clone as 67272-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67272-1-Ig (GZMK antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67272-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 67272-1-Ig (same clone as 67272-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67272-1-Ig (GZMK antibody) at dilution of 1:1000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67272-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 67272-1-Ig (same clone as 67272-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67272-1-Ig (Granzyme K antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67272-1-PBS in a different storage buffer formulation.

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  IF Staining of human tonsillitis using 67272-1-Ig (same clone as 67272-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using Granzyme K antibody (67272-1-Ig, Clone: 1C3A4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). DAPI (blue). This data was developed using the same antibody clone with 67272-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67272-1-PBS bindet in IHC, IF-P, ELISA Granzyme K und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Granzyme K fusion protein Ag27297
• Vollständiger Name: granzyme K (granzyme 3; NK tryptase II)
• Berechnetes Molekulargewicht: 29 kDa
• GenBank-Zugangsnummer: BC035802
• Gene symbol: GZMK
• Gene ID (NCBI): 3003
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Granzymes are a family of serine proteases stored in granules inside cytotoxic cells of the immune system. There are five human granzymes (granzyme A (GrA), GrB, GrH, GrK and GrM) currently identified, whereas mice have ten known granzymes (GrA-G, GrK, GrM and GrN) (PMID:14499263). Human GrK was first discovered in 1988 after purification from human peripheral blood mononuclear cells. GrK is expressed by cytotoxic T lymphocytes, natural killer T cells (NKT), γδ T cells and CD56bright+ NK cells.