GSDMD Polyklonaler Antikörper

GSDMD Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus und mehr (1)

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 30695-1-AP

Synonyme

AW558049, DF5L, Dfna5l, gasdermin D, Gsdmdc1

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  WB analysis using 30695-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30695-1-AP (Gsdmd antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis using 30695-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30695-1-AP (Gsdmd antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 30695-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 30695-1-AP (Gsdmd antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of RAW 264.7 using 30695-1-AP

  IP result of anti-Gsdmd (IP:30695-1-AP, 4ug; Detection:30695-1-AP 1:4000) with RAW 264.7 cells lysate 1400 ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: EL-4 cells, Mauslebergewebe, NIH/3T3-Zellen
• Erfolgreiche IP: RAW 264.7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

30695-1-AP bindet in WB, IP, ELISA GSDMD und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GSDMD fusion protein Ag33609
• Vollständiger Name: gasdermin D
• Berechnetes Molekulargewicht: 53 kDa
• Beobachtetes Molekulargewicht: 50-53 kDa, 43 kDa
• GenBank-Zugangsnummer: BC029813
• Gene symbol: Gsdmd
• Gene ID (NCBI): 69146
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GSDMD is a 53 kDa cytosolic protein and a member of the gasdermin protein family, which features 6 members in humans (GSDMA, -B, -C, -D, -E and -F. GSDMD was identified as the executioner of inflammasome-associated pyroptosis. Caspase-1, -11, or -4 cleave GSDMD after an aspartate residue within a central linker domain (D275 in humans and D276 in mice) to generate a 31-kDa N-terminal GSDMD fragment (GSDMD-NT) and a 22-kDa C-terminal fragment (GSDMD-CT). In addition to the canonical 31/22 kDa cleavage products, a 43 kDa fragment has been observed under specific experimental or pathological conditions. The generation of the 43 kDa fragment was observed upon caspase-3 and -7 activation during apoptosis. This antibody can detect 53 kDa full-length GSDMD and 43 kDa fragment as described in the literature (PMID: 28392147, PMID: 28979266).

Publikationen

Species	Application	Title
mouse	WB	Ecotoxicol Environ Saf; MANF inhibits NLRP3 inflammasome activation by competitively binding to DDX3X in paraquat-stimulated alveolar macrophages; Authors - Yi Pu
human	WB	Front Immunol; Comprehensive multiomics analysis identifies PYCARD as a key pyroptosis-related gene in osteoarthritis synovial macrophages; Authors - Zihao Yao