Histone H1.0 Polyklonaler Antikörper

Histone H1.0 Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, IP, ChIP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IF-P, IP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen(16)

Kat-Nr. : 17510-1-AP

Synonyme

H1F0, Histone H1, H1FV, H1-0, H10

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  WB analysis of HeLa using 17510-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17510-1-AP (Histone H1.0 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat spleen using 17510-1-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 17510-1-AP (Histone H1.0 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 17510-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17510-1-AP (H1F0 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 17510-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17510-1-AP (H1F0 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 17510-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 17510-1-AP (Histone H1.0 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 17510-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 17510-1-AP (H1F0 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A431 using 17510-1-AP

  IP result of anti-Histone H1.0 (IP:17510-1-AP, 4ug; Detection:17510-1-AP 1:500) with A431 cells lysate 2400ug.

• 

  IHC staining of human skin cancer using 17510-1-AP

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 17510-1-AP (Histone H1.0 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 17510-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 17510-1-AP (Histone H1.0 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse liver using 17510-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Histone H1.0 antibody (17510-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 17510-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Histone H1.0 antibody (17510-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  ChIP experiment of HeLa using 17510-1-AP

  Chromatin was prepared from HeLa cells, cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Histone H1.0 Antibody (17510-1-AP) or 5 ug of Normal Rabbit IgG (30000-0-AP), and 30 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real time PCR. Primers are located in the first kb of the transcribed region.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A431-Zellen, Jurkat-Zellen, Mausmilzgewebe, Rattenmilzgewebe
• Erfolgreiche IP: A431-Zellen
• Erfolgreiche Detektion in IHC: skin cancer; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Mauslebergewebe
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen
• Erfolgreiche ChIP: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• CHImmunpräzipitation (IP): CHIP : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 15 publications below
• IF: See 2 publications below
• ChIP: See 1 publications below

Produktinformation

17510-1-AP bindet in WB, IHC, IF/ICC, IF-P, IP, ChIP, ELISA Histone H1.0 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Histone H1.0 fusion protein Ag9982
• Vollständiger Name: H1 histone family, member 0
• Berechnetes Molekulargewicht: 21 kDa
• Beobachtetes Molekulargewicht: 32 kDa
• GenBank-Zugangsnummer: BC000145
• Gene symbol: Histone H1.0
• Gene ID (NCBI): 3005
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.Linker histones are involved in the formation of higher order structure in chromatin and the maintenance of overall chromatin compaction. The H1F0 histones are found in cells that are in terminal stages of differentiation or that have low rates of cell division.Histone H1.0 (H1F0,H1FV) is a linker histone that is widely expressed in many tissues and almost all vertebrates, unlike some other linker histones. The observed molecular weight of H1F0 is about 32 kDa.

Publikationen

Species	Application	Title
mouse	WB	Nat Neurosci; Aerobic glycolysis is the predominant means of glucose metabolism in neuronal somata, which protects against oxidative damage; Authors - Yao Wei
human	WB	Theranostics; PMID: 29290796; Authors - Jin Zeng
human	WB	Nucleic Acids Res; Pin1 promotes GR transactivation by enhancing recruitment to target genes.; Authors - Poolman Toryn M TM
human	WB,IF	Oncogene; Downregulation of HINFP induces senescence-associated secretory phenotype to promote metastasis in a non-cell-autonomous manner in bladder cancer.; Authors - Xianchong Zheng
human	WB	PLoS Pathog; Human cytomegalovirus UL23 inhibits transcription of interferon-γ stimulated genes and blocks antiviral interferon-γ responses by interacting with human N-myc interactor protein.; Authors - Linyuan Feng
human	WB	Front Cell Dev Biol; GATA4 Regulates Inflammation-Driven Pancreatic Ductal Adenocarcinoma Progression.; Authors - Weiliang Jiang