H2AFY Polyklonaler Antikörper

H2AFY Polyklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF

Konjugation

Unkonjugiert

Kat-Nr. : 26875-1-AP

Synonyme

Core histone macro H2A.1, H2A histone family, member Y, H2A.y, H2A/y, H2AF12M, H2AFJ, H2AFY, Histone H2A.y, Histone macroH2A1, MACROH2A1, MACROH2A1.1, macroH2A1.2, mH2A1

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Galerie der Validierungsdaten

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  WB analysis using 26875-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26875-1-AP (H2AFY antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 26875-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 26875-1-AP (H2AFY Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Caco-2 using 26875-1-AP

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 26875-1-AP (H2AFY Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 26875-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 26875-1-AP (H2AFY Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 26875-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 26875-1-AP (H2AFY Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 26875-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using H2AFY antibody (26875-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, Caco-2-Zellen, HeLa-Zellen, Maushirngewebe
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

26875-1-AP bindet in WB, IF H2AFY und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: H2AFY fusion protein Ag25445
• Vollständiger Name: H2A histone family, member Y
• Berechnetes Molekulargewicht: 372 aa, 40 kDa
• Beobachtetes Molekulargewicht: 40 kDa
• GenBank-Zugangsnummer: BC013331
• Gene symbol: H2AFY
• Gene ID (NCBI): 9555
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.