Histone H2A.z Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Histone H2A.z Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

Kat-Nr. : 16441-1-AP

Synonyme

H2AFZ, Histone H2A, H2AZ1, H2AZ, H2A/z

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  WB analysis of human brain using 16441-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16441-1-AP (H2AFZ antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 16441-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16441-1-AP (Histone H2A.z Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16441-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16441-1-AP (H2AFZ antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skin cancer using 16441-1-AP

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 16441-1-AP (Histone H2A.z antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 16441-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 16441-1-AP (Histone H2A.z antibody) at dilution of 1:200 (under 10x lens).

• 

  IF Staining of HeLa using 16441-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 16441-1-AP (Histone H2A.z antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Hirngewebe, Mausnierengewebe
• Erfolgreiche Detektion in IHC: humanes Hautkrebsgewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IF: See 3 publications below

Produktinformation

16441-1-AP bindet in WB, IHC, IF/ICC, ELISA Histone H2A.z und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Fisch, Maus, Oratosquilla oratoria
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Histone H2A.z fusion protein Ag9762
• Vollständiger Name: H2A histone family, member Z
• Berechnetes Molekulargewicht: 128 aa, 14 kDa
• Beobachtetes Molekulargewicht: 14 kDa
• GenBank-Zugangsnummer: BC018002
• Gene symbol: Histone H2A.z
• Gene ID (NCBI): 3015
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
fish	IF	Sci Adv; The lungfish cocoon is a living tissue with antimicrobial functions.; Authors - Ryan Darby Heimroth
mouse	WB	J Biol Chem; The chromatin remodeler protein Chd4 maintains embryonic stem cell identity by controlling pluripotency- and differentiation-associated genes.; Authors - Haixin Zhao ; KD Validated
human	WB	Biomed Pharmacother; Limonin enhances the radiosensitivity of nasopharyngeal carcinoma cells via attenuating Stat3-induced cell stemness.; Authors - Ling Gao
mouse	IF,WB	Biol Open; Loss of Elp1 perturbs histone H2A.Z and the Notch signaling pathway.; Authors - BreAnna Cameron
Oratosquilla oratoria	IF	J Morphol; Ultrastructure of spermiogenesis and the distribution of spermatozoal nuclear histones in the Japanese mantis shrimp, Oratosquilla oratoria (Crustacea: Stomatopoda).; Authors - Tingrong Chen
human	WB	Front Oncol; DJ-1 promotes osteosarcoma progression through activating CDK4/RB/E2F1 signaling pathway; Authors - Zhitao Han