H3F3B Polyklonaler Antikörper

H3F3B Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10265-1-AP

Synonyme

H3 histone, family 3B (H3.3B), H3.3A, H3.3B, H3F3, H3F3A, H3F3B, histone, Histone H3.3

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 10265-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10265-1-AP (H3F3B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10265-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10265-1-AP (H3F3B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

10265-1-AP bindet in WB, ELISA H3F3B und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: H3F3B fusion protein Ag0326
• Vollständiger Name: H3 histone, family 3B (H3.3B)
• Berechnetes Molekulargewicht: 15 kDa
• Beobachtetes Molekulargewicht: 17 kDa
• GenBank-Zugangsnummer: BC001124
• Gene symbol: H3F3B
• Gene ID (NCBI): 3021
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Histones are the basic nuclear proteins responsible for the nucleosome structure of the chromosomal fiber. Five classes of histone genes have been reported, some of which are expressed only during S phase, while others are replication independent. The latter are referred to as replacement histones and are expressed in quiescent or terminally differentiated cells. H3F3B is one replacement histone [PMID: 8586426]. It constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Thereby it has a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability [PMID: 14718166, 15776021].