Phospho-Histone H3 (Ser10) Monoklonaler Antikörper

Phospho-Histone H3 (Ser10) Monoklonal Antikörper für WB, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human und mehr (3)

Anwendung

WB, IHC, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(23)

CloneNo.

4C7G2

Rezensionen (3)

Kat-Nr. : 66863-1-Ig

Synonyme

H3 P-S10, H3S10, H3S10Ph, Histone H3 (Ser10), p Histone H3

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  WB analysis using 66863-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66863-1-Ig (Phospho-Histone H3 (Ser10) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis using 66863-1-Ig

  Non-treated and Calyculin A treated cells were subjected to SDS PAGE followed by western blot with 66863-1-Ig (Phospho-Histone H3 (Ser10) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  IHC staining of Jurkat using 66863-1-Ig

  Immunohistochemical analysis of paraffin-embedded Jurkat cells slide using 66863-1-Ig (Phospho-Histone H3 (Ser10) antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of C2C12 using 66863-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using Phospho-Histone H3 (Ser10) antibody (66863-1-Ig, Clone: 4C7G2 ) at dilution of 1:1200 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), Alpha Tubulin antibody (11224-1-AP, green).

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  IF Staining of HeLa using 66863-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Phospho-Histone H3 (Ser10) antibody (66863-1-Ig, Clone: 4C7G2 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), Beta Tubulin antibody (80713-1-RR, Clone: 2O13, red).

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  IF Staining of HeLa using 66863-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Phospho-Histone H3 (Ser10) antibody (66863-1-Ig, Clone: 4C7G2 ) at dilution of 1:1500 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), Alpha Tubulin antibody (11224-1-AP, green).

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  IF Staining of MCF-7 using 66863-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Phospho-Histone H3 (Ser10) antibody (66863-1-Ig, Clone: 4C7G2 ) at dilution of 1:1500 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), Alpha Tubulin antibody (11224-1-AP, green).

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  FC experiment of HeLa using 66863-1-Ig

  1x10^6 nocodazole treated HeLa cells were intracellularly stained with 0.25 ug Phospho-Histone H3 (Ser10) Monoclonal antibody (66863-1-Ig, Clone:4C7G2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), and 0.25 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, Jurkat-Zellen
• Erfolgreiche Detektion in IF/ICC: C2C12-Zellen, HeLa-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in FC (Intra): nocodazole treated HeLa cells

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:600-1:2400
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 10 publications below
• IHC: See 3 publications below
• IF: See 11 publications below

Produktinformation

66863-1-Ig bindet in WB, IHC, IF/ICC, FC (Intra), ELISA Phospho-Histone H3 (Ser10) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Huhn, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: histone cluster 1, H3a
• Berechnetes Molekulargewicht: 15 kDa
• Beobachtetes Molekulargewicht: 15-17 kDa
• GenBank-Zugangsnummer: NM_003529
• Gene symbol: HIST1H3A
• Gene ID (NCBI): 8350
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Phospho-histone-H3 (PHH3) is a core histone protein, which in its phosphorylated state forms the principal constituents of eukaryotic chromatin, with histone H3 being phosphorylated at serine (Ser) 10 or Ser28 as well as its phosphorylation of Ser10 being strongly correlated with the late G2 to M-phase transition in mammalian mitotic cells. On the basis of previous research, a few cell line- and animal model-based researches have displayed an increase in phosphorylation of histone H3 at Ser10 (H3S10ph), the only histone marker that is involved in carcinogenesis and cellular transformation. Histone H3 phosphorylation on serine-10 is specific to mitosis and phosphorylated histone H3 (PHH3) proliferation markers (as counts defined per area or as indices defined per cell numbers) are increasingly being used to evaluate proliferation in various tumors.

Publikationen

Species	Application	Title
human	IHC	Oncogene; Oncogenic long intervening noncoding RNA Linc00284 promotes c-Met expression by sponging miR-27a in colorectal cancer.; Authors - Jun You
	IF	EMBO Rep; Palmitoylated importin α regulates mitotic spindle orientation through interaction with NuMA; Authors - Patrick James Sutton
	IF	iScience; Centrosomal protein 120 promotes centrosome amplification and gastric cancer progression via USP54-mediated deubiquitination of PLK4; Authors - Chenggang Zhang
human	WB	Biochem Pharmacol; A novel aromatic amide derivative SY-65 co-targeted tubulin and histone deacetylase 1 with potent anticancer activity in vitro and in vivo.; Authors - Yin-Ru Li
human	IF	Front Mol Biosci; High-Resolution Imaging of Tumor Spheroids and Organoids Enabled by Expansion Microscopy.; Authors - Steven J Edwards
mouse	IF	Sci Rep; NF90-NF45 is essential for β cell compensation under obesity-inducing metabolic stress through suppression of p53 signaling pathway.; Authors - Sylvia Lai

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Charlotte (Verified Customer) (09-19-2024)

Blocking and dilution in seablock. Antibody very strong given the dilution and the bands intensity. Very satisfied.

• Applications: Western Blot
• Primary Antibody Dilution: 1/10000
• Cell Tissue Type: HELA

Phospho-Histone H3 (Ser10) Antibody Western Blot validation (1/10000 dilution) in HELA (Cat no:66863-1-Ig)

FH

Tsimafei (Verified Customer) (08-04-2024)

Membrane was incubated at 4C, ON

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: mESCs

Phospho-Histone H3 (Ser10) Antibody Western Blot validation (1:3000 dilution) in mESCs (Cat no:66863-1-Ig)

FH

Wissem (Verified Customer) (06-27-2024)

This antibody works great at detecting phosphorylation of Histone H3 at S10; conditions - 1:10,000 overnight at 4C; used to assess cell cycle synchronisation in n extracts from U2OS, HCT116, HeLa, RPE1 and HEK293 cells

• Applications: Western Blot
• Primary Antibody Dilution: 1:10,000