HA Tag Monoklonaler Antikörper

HA Tag Monoklonal Antikörper für IF/ICC

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

rekombinanten Protein

Anwendung

IF/ICC

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Publikationen(2)

CloneNo.

1F5C6

Kat-Nr. : CL488-66006

Synonyme

YPYDVPDYA

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  IF Staining of Transfected HEK-293 using CL488-66006

  Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using CoraLite® Plus 488 HA Tag antibody (CL488-66006, Clone: 1F5C6 ) at dilution of 1:200.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: Transfizierte HEK-293-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

CL488-66006 bindet in IF/ICC HA Tag und zeigt Reaktivität mit rekombinanten Protein

• Getestete Reaktivität: rekombinanten Protein
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: HA Tag
• Gene symbol: HA Tag
• Gene ID (NCBI): 6
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Protein tags are a protein or peptide sequences located either on the C- or N- terminal of the target protein, which can facilitate solubility, detection, purification, localization, and expression of the target protein. The HA tag corresponds to amino acid residues YPYDVPDYA of a surface glycoprotein -human influenza virus hemagglutinin (HA). The HA tag is commonly used for a variety of research applications including chromatin immunoprecipitation, ELISA, flow cytometry, western blotting, immunocytochemistry/immunofluorescence among of others.

Publikationen

Species	Application	Title
	IF	Cell Rep; Hypoxia-sensitive cells trigger NK cell activation via the KLF4-ASH1L-ICAM-1 axis, contributing to impairment in the rat epididymis; Authors - Jitao Zeng
		J Extracell Vesicles; PlexinA1 (PLXNA1) as a novel scaffold protein for the engineering of extracellular vesicles; Authors - Hang Zhao