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HA Tag Rekombinanter Antikörper

HA Tag Rekombinant Antikörper für IF/ICC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

rekombinanten Protein

Anwendung

IF/ICC

Konjugation

CoraLite®647 Fluorescent Dye

CloneNo.

6I21

Kat-Nr. : CL647-81290

Synonyme

HA

Filter:
  • Immunofluorescence (IF) / fluorescent staining of Transfected HEK-293 cells using CoraLite®647-conjugated HA Tag Recombinant antibod (CL647-81290)

    IF Staining of Transfected HEK-293 using CL647-81290

    Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using CoraLite®647 HA Tag antibody (CL647-81290, Clone: 6I21 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of Transfected HEK-293 cells using CoraLite®647-conjugated HA Tag Recombinant monoclo (CL647-81290)

    FC experiment of Transfected HEK-293 using CL647-81290

    1x10^6 Transfected HEK-293 cells were intracellularly stained with 0.4 ug CoraLite®647-conjugated HA Tag Recombinant monoclonal antibody (CL647-81290, Clone:6I21), and 0.4 ug CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00017).

Geprüfte Anwendungen

Erfolgreiche Detektion in IF/ICCTransfizierte HEK-293-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL647-81290 bindet in IF/ICC HA Tag und zeigt Reaktivität mit rekombinanten Protein

Getestete Reaktivität rekombinanten Protein
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen Peptid
Vollständiger Name HA Tag
Gene symbol HA Tag
Gene ID (NCBI) 6
Konjugation CoraLite®647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Protein tags are a protein or peptide sequences located either on the C- or N- terminal of the target protein, which can facilitate solubility, detection, purification, localization, and expression of the target protein. The HA tag corresponds to amino acid residues YPYDVPDYA of a surface glycoprotein -human influenza virus hemagglutinin (HA). The HA tag is commonly used for a variety of research applications including chromatin immunoprecipitation, ELISA, flow cytometry, western blotting, immunocytochemistry/immunofluorescence among of others.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
IF protocol for CL647 HA Tag antibody CL647-81290Protokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Parijat (Verified Customer) (03-06-2024)

Good antibody, saves a lot of time since its already conjugated.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:300
  • Cell Tissue Type: 293T cells