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HA Tag Rekombinanter Antikörper

HA Tag Rekombinant Antikörper für WB

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

rekombinanten Protein

Anwendung

WB

Konjugation

HRP Fluorescent Dye

CloneNo.

6I21

Kat-Nr. : HRP-81290

Synonyme

Galerie der Validierungsdaten

Filter:
  • Western Blot (WB) analysis of Transfected HEK-293T cells using HRP-conjugated HA Tag Recombinant antibody (HRP-81290)

    WB analysis of Transfected HEK-293T using HRP-81290

    Non-transfected and transfected HEK-293T lysates were subjected to SDS PAGE followed by western blot with HRP-81290 (HA tagged antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Geprüfte Anwendungen

Erfolgreiche Detektion in WBTransfected HEK-293T cells

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:5000-1:50000
Sample-dependent, check data in validation data gallery

Produktinformation

HRP-81290 bindet in WB HA Tag und zeigt Reaktivität mit rekombinanten Protein

Getestete Reaktivität rekombinanten Protein
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen Peptid
Vollständiger Name HA Tag
Gene symbol
Gene ID (NCBI)
Konjugation HRP Fluorescent Dye
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Protein tags are a protein or peptide sequences located either on the C- or N- terminal of the target protein, which can facilitate solubility, detection, purification, localization, and expression of the target protein. The HA tag corresponds to amino acid residues YPYDVPDYA of a surface glycoprotein -human influenza virus hemagglutinin (HA). The HA tag is commonly used for a variety of research applications including chromatin immunoprecipitation, ELISA, flow cytometry, western blotting, immunocytochemistry/immunofluorescence among of others.