HA Tag Rekombinanter Antikörper

HA Tag Rekombinant Antikörper für WB

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

rekombinanten Protein

Anwendung

WB, IP, CoIP

Konjugation

HRP

Publikationen(10)

CloneNo.

6I21

Kat-Nr. : HRP-81290

Synonyme

HA

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  WB analysis of Transfected HEK-293T using HRP-81290

  Non-transfected and transfected HEK-293T lysates were subjected to SDS PAGE followed by western blot with HRP-81290 (HA tagged antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Transfected HEK-293T cells

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 10 publications below
• IP: See 3 publications below
• CoIP: See 1 publications below

Produktinformation

HRP-81290 bindet in WB, IP, CoIP HA Tag und zeigt Reaktivität mit rekombinanten Protein

• Getestete Reaktivität: rekombinanten Protein
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: HA Tag
• Gene symbol: HA Tag
• Gene ID (NCBI): 6
• Konjugation: HRP
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Protein tags are a protein or peptide sequences located either on the C- or N- terminal of the target protein, which can facilitate solubility, detection, purification, localization, and expression of the target protein. The HA tag corresponds to amino acid residues YPYDVPDYA of a surface glycoprotein -human influenza virus hemagglutinin (HA). The HA tag is commonly used for a variety of research applications including chromatin immunoprecipitation, ELISA, flow cytometry, western blotting, immunocytochemistry/immunofluorescence among of others.

Publikationen

Species	Application	Title
	WB	Cell Death Dis; USP3 promotes DNA damage response and chemotherapy resistance through stabilizing and deubiquitinating SMARCA5 in prostate cancer; Authors - Sheng Li
	WB	J Virol; RNASEK interacting with PEDV structural proteins facilitates virus entry via clathrin-mediated endocytosis; Authors - Wenzhen Qin
	WB,IP	Theranostics; USP14 modulates stem-like properties, tumorigenicity, and radiotherapy resistance in glioblastoma stem cells through stabilization of MST4-phosphorylated ALKBH5; Authors - Xiao Zhou
	WB,IP	PLoS Genet; Evolution of canonical circadian clock genes underlies unique sleep strategies of marine mammals for secondary aquatic adaptation; Authors - Daiqing Yin
	WB	J Virol; Midnolin inhibits coronavirus proliferation by degrading viral proteins; Authors - Yahe Wang
	WB	Int J Mol Sci; CircMTA2 Drives Gastric Cancer Progression through Suppressing MTA2 Degradation via Interacting with UCHL3; Authors - Gengchen Xie