HA tag Polyklonaler Antikörper

HA tag Polyklonal Antikörper für WB, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

rekombinanten Protein und mehr (4)

Anwendung

WB, IF/ICC, FC (Intra), IP, CoIP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen(1084)

Rezensionen (18)

Kat-Nr. : 51064-2-AP

Synonyme

HA, YPYDVPDYA

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• Validation Data Gallery
• Proteintech vs. Other Vendors

• 

  WB analysis of recombinant protein using 51064-2-AP

  Western blot of HA-tagged fusion protein with anti-HA-tag (51064-2-AP) at various dilutions.

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  WB analysis of Transfected HEK-293 using 51064-2-AP

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 51064-2-AP (HA tag antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Transfected HEK-293 using 51064-2-AP

  IP result of anti-HA tag (IP:51064-2-AP, 4ug; Detection:51064-2-AP 1:20000) with Transfected HEK-293 cells lysate 1280 ug.

• 

  IF Staining of Transfected HEK-293 using 51064-2-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using HA tag antibody (51064-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  IF Staining of Transfected HEK-293 using 51064-2-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using HA tag antibody (51064-2-AP) at dilution of 1:200 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of Transfected HEK-293 using 51064-2-AP

  1x10^6 Transfected HEK-293 cells were intracellularly stained with 0.25 ug HA tag Polyclonal antibody (51064-2-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), and 0.25 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: recombinant protein, Transfizierte HEK-293-Zellen
• Erfolgreiche IP: Transfizierte HEK-293-Zellen
• Erfolgreiche Detektion in IF/ICC: Transfizierte HEK-293-Zellen
• Erfolgreiche Detektion in FC (Intra): Transfizierte HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 777 publications below
• IF: See 69 publications below
• IP: See 222 publications below
• ELISA: See 1 publications below
• CoIP: See 85 publications below
• ChIP: See 10 publications below

Produktinformation

51064-2-AP bindet in WB, IF/ICC, FC (Intra), IP, CoIP, ChIP, ELISA HA tag und zeigt Reaktivität mit rekombinanten Protein

• Getestete Reaktivität: rekombinanten Protein
• In Publikationen genannte Reaktivität: human, Affe, Hausschwein, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: HA tag
• Berechnetes Molekulargewicht: 1 kDa
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The HA tag is corresponds to amino acid residues YPYDVPDYA of human influenza virus hemagglutinin(HA). Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. The HA tag is useful in western blotting and immunohistochemical localization of expressed fusion proteins when examined with antibodies raised specifically against the HA-tag.

Publikationen

Species	Application	Title
	WB	Nature; 7-Dehydrocholesterol dictates ferroptosis sensitivity; Authors - Yaxu Li
	WB	Signal Transduct Target Ther; FBXW7β loss-of-function enhances FASN-mediated lipogenesis and promotes colorectal cancer growth; Authors - Wenxia Wei
	WB,CoIP	Signal Transduct Target Ther; Circulating tumor cells shielded with extracellular vesicle-derived CD45 evade T cell attack to enable metastasis; Authors - Chuan Yang
	WB	Cell; RetroCHMP3 blocks budding of enveloped viruses without blocking cytokinesis.; Authors - Lara Rheinemann
	WB	Cell; Glycogen accumulation and phase separation drives liver tumor initiation.; Authors - Qingxu Liu
	WB	Gastroenterology; PTEN deficiency facilitates exosome secretion and metastasis in cholangiocarcinoma by impairing TFEB-mediated lysosome biogenesis; Authors - Tian-Yi Jiang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kiara (Verified Customer) (07-28-2025)

This primary antibody did give a signal for HA-tagged dCas9-KRAB in our CRISPRi iPSC-CMs, but however it also gave quite some background in our WTC11 negative control line.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: iPSC-derived cardiomyocytes

HA tag Antibody Immunofluorescence validation (1:50 dilution) in iPSC-derived cardiomyocytes (Cat no:51064-2-AP)

FH

Elizabeth (Verified Customer) (07-24-2025)

Our lab mainly purchase Proteintech antibodies. They function well; minimal background "noise". We will continue to purchase these antibodies

• Applications: Western Blot, Immunohistochemistry, Immunoprecipitation
• Cell Tissue Type: Mainly mammalian tissue

FH

Tiffany (Verified Customer) (05-27-2025)

Very good quality. We dilute this HA antibody in 3% BSA solution and use it as primary antibody for western blot. This mixture can be reused for up to 3 months.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000

FH

Tiffany (Verified Customer) (01-06-2025)

The quality is as good as our previous purchase.

• Applications: Western Blot

FH

PK (Verified Customer) (07-05-2024)

eXCELLENT

• Applications: Western Blot
• Primary Antibody Dilution: 1000
• Cell Tissue Type: HEK

HA tag Antibody Western Blot validation (1000 dilution) in HEK (Cat no:51064-2-AP)

FH

S (Verified Customer) (05-16-2024)

Excellent

• Applications: Western Blot
• Primary Antibody Dilution: 2000
• Cell Tissue Type: AC16

HA tag Antibody Western Blot validation (2000 dilution) in AC16 (Cat no:51064-2-AP)

FH

manohar (Verified Customer) (07-10-2023)

Used Nitrocellulose membrane with 5% milk as blocking for 1 hr, 1% milk used for antibody dilution and kept for overnight incubation

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293, Neuronal stem cells

FH

X (Verified Customer) (07-11-2022)

Good for both WB and IF in transfected cells

• Applications: Western Blot, Immunohistochemistry
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Transfected N2A cells

FH

Victor (Verified Customer) (06-13-2022)

I'm using the antibody for Western blotting tissue culture samples and it works well for my needs for the price. I've been using a 1:2000 dilution which gives me decent bands via Western blot and analyzed using a fluorescent anti-rabbit secondary. The bands could be a little brighter, but this antibody is worth it for the sale price. It's stored in glycerol, so it saves you the trouble of aliquoting and it can be kept at -20 which is nice.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000

FH

Nick (Verified Customer) (04-20-2022)

The antibody works well and performs consistently in IP and WB

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000

FH

Deyong (Verified Customer) (03-11-2022)

Overall it worked good in the HEK293T cells with protein overexpression, but there were multi non specific bands below.

• Applications: Western Blot
• Primary Antibody Dilution: 1/3000
• Cell Tissue Type: HEK293T cells

FH

Stephanie (Verified Customer) (01-28-2022)

Antibody easy to use, right recommended dilution. No background observed and clean bands.

• Applications: Western Blot
• Primary Antibody Dilution: 1000

FH

S (Verified Customer) (11-22-2021)

Overall, the antibody worked well, but there was a strong band in my control (negative) lane.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: HEK293T

HA tag Antibody Western Blot validation (1:2000 dilution) in HEK293T (Cat no:51064-2-AP)

FH

K (Verified Customer) (07-15-2021)

This ab recognized the HA tag-ed proteins, but also got a lot of non-specific bands, dilution used were 1:2000

• Applications: Western Blot
• Cell Tissue Type: 293T

FH

Fen (Verified Customer) (03-06-2020)

it works great for both western blot and IP

• Applications: Western Blot, Immunoprecipitation,
• Cell Tissue Type: B cells

FH

Chun (Verified Customer) (12-05-2019)

An excellent antibody

• Applications: Western Blot,
• Primary Antibody Dilution: 1:5,000
• Cell Tissue Type: HEK293 cells

FH

Nikhil (Verified Customer) (10-16-2019)

HA tagged VprBP protein was expressed in H1299 cells. Blue: DAPI; Green:HA-VprBP

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: H1299

HA tag Antibody Immunofluorescence, validation (1:100 dilution) in H1299 (Cat no:51064-2-AP)

FH

Phuong (Verified Customer) (06-19-2019)

HA tag polyclonal antibody works very well. Gives very clean and strong signal.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: 293T