HA tag Polyklonaler Antikörper

HA tag Polyklonal Antikörper für IF, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

rekombinanten Protein und mehr (8)

Anwendung

WB, IP, IHC, IF, FC, CoIP, ChIP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 51064-2-AP

Synonyme

HA tag, YPYDVPDYA


Filter:
  • Western Blot (WB) analysis of recombinant protein using HA tag Polyclonal antibody (51064-2-AP)

    WB analysis of recombinant protein using 51064-2-AP

    Western blot of HA-tagged fusion protein with anti-HA-tag (51064-2-AP) at various dilutions.

  • Western Blot (WB) analysis of Transfected HEK-293 cells using HA tag Polyclonal antibody (51064-2-AP)

    WB analysis of Transfected HEK-293 using 51064-2-AP

    Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 51064-2-AP (HA tag antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Transfected HEK-293 cells using HA tag Polyclonal antibody (51064-2-AP)

    IP experiment of Transfected HEK-293 using 51064-2-AP

    IP Result of anti-HA tag (IP:51064-2-AP, 3ug; Detection:51064-2-AP 1:2000) with Transfected HEK-293 cells lysate 500ug.

  • Immunofluorescence (IF) / fluorescent staining of Transfected HEK-293 cells using HA tag Polyclonal antibody (51064-2-AP)

    IF Staining of Transfected HEK-293 using 51064-2-AP

    Immunofluorescent analysis of Transfected HEK-293 cells using 51064-2-AP (HA tag antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of Transfected HEK-293 cells using HA tag Polyclonal antibody (51064-2-AP)

    IF Staining of Transfected HEK-293 using 51064-2-AP

    Immunofluorescent analysis of Transfected HEK-293 cells using 51064-2-AP (HA tag antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of Transfected HEK-293 cells using HA tag Polyclonal antibody (51064-2-AP)

    IF Staining of Transfected HEK-293 using 51064-2-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using HA tag antibody (51064-2-AP) at dilution of 1:200 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

"HA tag Antibodies" Comparison

View side-by-side comparison of HA tag antibodies from other vendors to find the one that best suits your research needs.

Geprüfte Anwendungen

Erfolgreiche Detektion in WBrecombinant protein, Transfizierte HEK-293-Zellen
Erfolgreiche IPTransfizierte HEK-293-Zellen
Erfolgreiche Detektion in IFTransfizierte HEK-293-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:5000-1:10000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunfluoreszenz (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

51064-2-AP bindet in WB, IP, IHC, IF, FC, CoIP, ChIP, ELISA HA tag und zeigt Reaktivität mit rekombinanten Protein

Getestete Reaktivität rekombinanten Protein
In Publikationen genannte Reaktivitäthuman, Affe, Ente, Huhn, Hund, Maus, Ratte, Zebrafisch
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen Peptid
Vollständiger Name HA tag
Berechnetes Molekulargewicht 1 kDa
Gene symbol
Gene ID (NCBI)
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The HA tag is corresponds to amino acid residues YPYDVPDYA of human influenza virus hemagglutinin(HA). Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. The HA tag is useful in western blotting and immunohistochemical localization of expressed fusion proteins when examined with antibodies raised specifically against the HA-tag.

Protokolle

Produktspezifische Protokolle
WB protocol for HA tag antibody 51064-2-APProtokoll herunterladen
IF protocol for HA tag antibody 51064-2-APProtokoll herunterladen
IP protocol for HA tag antibody 51064-2-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Signal Transduct Target Ther

FBXW7β loss-of-function enhances FASN-mediated lipogenesis and promotes colorectal cancer growth

Authors - Wenxia Wei
WB

Cell

RetroCHMP3 blocks budding of enveloped viruses without blocking cytokinesis.

Authors - Lara Rheinemann
WB

Cell

Glycogen accumulation and phase separation drives liver tumor initiation.

Authors - Qingxu Liu
humanWB

Gastroenterology

PTEN deficiency facilitates exosome secretion and metastasis in cholangiocarcinoma by impairing TFEB-mediated lysosome biogenesis

Authors - Tian-Yi Jiang
WB

Cell

The Human RNA-Binding Proteome and Its Dynamics during Translational Arrest.

Authors - Jakob Trendel
WB

Nat Genet

Pathogenic SPTBN1 variants cause an autosomal dominant neurodevelopmental syndrome.

Authors - Margot A Cousin

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

manohar (Verified Customer) (07-10-2023)

Used Nitrocellulose membrane with 5% milk as blocking for 1 hr, 1% milk used for antibody dilution and kept for overnight incubation

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293, Neuronal stem cells
FH

X (Verified Customer) (07-11-2022)

Good for both WB and IF in transfected cells

  • Applications: Western Blot, Immunohistochemistry
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: Transfected N2A cells
FH

Victor (Verified Customer) (06-13-2022)

I'm using the antibody for Western blotting tissue culture samples and it works well for my needs for the price. I've been using a 1:2000 dilution which gives me decent bands via Western blot and analyzed using a fluorescent anti-rabbit secondary. The bands could be a little brighter, but this antibody is worth it for the sale price. It's stored in glycerol, so it saves you the trouble of aliquoting and it can be kept at -20 which is nice.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
FH

Nick (Verified Customer) (04-20-2022)

The antibody works well and performs consistently in IP and WB

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
FH

Deyong (Verified Customer) (03-11-2022)

Overall it worked good in the HEK293T cells with protein overexpression, but there were multi non specific bands below.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/3000
  • Cell Tissue Type: HEK293T cells
FH

Stephanie (Verified Customer) (01-28-2022)

Antibody easy to use, right recommended dilution. No background observed and clean bands.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1000
FH

S (Verified Customer) (11-22-2021)

Overall, the antibody worked well, but there was a strong band in my control (negative) lane.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: HEK293T
HA tag Antibody Western Blot validation (1:2000 dilution) in HEK293T (Cat no:51064-2-AP)
FH

K (Verified Customer) (07-15-2021)

This ab recognized the HA tag-ed proteins, but also got a lot of non-specific bands, dilution used were 1:2000

  • Applications: Western Blot
  • Cell Tissue Type: 293T
FH

Fen (Verified Customer) (03-06-2020)

it works great for both western blot and IP

  • Applications: Western Blot, Immunoprecipitation,
  • Cell Tissue Type: B cells
FH

Chun (Verified Customer) (12-05-2019)

An excellent antibody

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:5,000
  • Cell Tissue Type: HEK293 cells
FH

Nikhil (Verified Customer) (10-16-2019)

HA tagged VprBP protein was expressed in H1299 cells. Blue: DAPI; Green:HA-VprBP

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: H1299
HA tag Antibody Immunofluorescence, validation (1:100 dilution) in H1299 (Cat no:51064-2-AP)
FH

Phuong (Verified Customer) (06-19-2019)

HA tag polyclonal antibody works very well. Gives very clean and strong signal.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:3000
  • Cell Tissue Type: 293T