HAAO Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

HAAO Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 12791-1-AP

Synonyme

EC:1.13.11.6, 3-hydroxyanthranilic acid dioxygenase, 3-hydroxyanthranilate oxygenase, 3-hydroxyanthranilate 3,4-dioxygenase, 3-HAO

---

• 

  WB analysis of human liver using 12791-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 12791-1-AP (HAAO antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 12791-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12791-1-AP (HAAO Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 12791-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 12791-1-AP (HAAO Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human gliomas using 12791-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12791-1-AP (HAAO Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human gliomas using 12791-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12791-1-AP (HAAO Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HepG2 using 12791-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 12791-1-AP (HAAO antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Lebergewebe, Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 2 publications below

Produktinformation

12791-1-AP bindet in WB, IHC, IF/ICC, ELISA HAAO und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HAAO fusion protein Ag3511
• Vollständiger Name: 3-hydroxyanthranilate 3,4-dioxygenase
• Berechnetes Molekulargewicht: 286 aa, 33 kDa
• Beobachtetes Molekulargewicht: 33 kDa
• GenBank-Zugangsnummer: BC029510
• Gene symbol: HAAO
• Gene ID (NCBI): 23498
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB,IHC	Adv Sci (Weinh); Tryptophan Metabolism Acts as a New Anti-Ferroptotic Pathway to Mediate Tumor Growth; Authors - Dong Liu ; KO Validated
rat	IHC	BMC Genomics; Proteomics and transcriptomics profiling reveals distinct aspects of kidney stone related genes in calculi rats; Authors - Wang Zhu
human	WB	Nat Commun; Niacin restriction with NAMPT-inhibition is synthetic lethal to neuroendocrine carcinoma; Authors - Miyuki Nomura
mouse	WB	Environ Pollut; Bisphenol F exposure induces depression-like changes: Roles of the kynurenine metabolic pathway along the "liver-brain" axis; Authors - Zheng Yu
mouse	WB	Gut Microbes; Gut-derived lactic acid enhances tryptophan to 5-hydroxytryptamine in regulation of anxiety via Akkermansia muciniphila; Authors - Miaomiao Pan