HACE1 Polyklonaler Antikörper

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• KD/KO Validated

HACE1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 24104-1-AP

Synonyme

HACE1, KIAA1320

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  WB analysis of HEK-293 using 24104-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24104-1-AP (HACE1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 24104-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24104-1-AP (HACE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 24104-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 24104-1-AP (HACE1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 24104-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 24104-1-AP (HACE1 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HeLa using 24104-1-AP

  Immunofluorescent analysis of HeLa cells using 24104-1-AP (HACE1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:100-1:400
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 2 publications below

Produktinformation

24104-1-AP bindet in WB, IHC, IF/ICC, ELISA HACE1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HACE1 fusion protein Ag21130
• Vollständiger Name: HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1
• Berechnetes Molekulargewicht: 909 aa, 102 kDa
• Beobachtetes Molekulargewicht: 78 kDa
• GenBank-Zugangsnummer: BC034982
• Gene symbol: HACE1
• Gene ID (NCBI): 57531
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HACE1(E3 ubiquitin-protein ligase HACE1) also named as KIAA1320, has a tumor-suppressor function dependent on its E3 ligase activity and controlling cell cycle progression during cell stress through degradation of cyclin D1. It belongs to the HECT family of E3 ubiqutin protein ligase. This protein has 4 isoforms produced by alternative splicing with the molecular weight of 102 kDa, 36 kDa, 64 kDa and 78 kDa.

Publikationen

Species	Application	Title
mouse	WB	Reprod Biol Endocrinol; NET1 is a critical regulator of spindle assembly and actin dynamics in mouse oocytes; Authors - Shiwei Wang ; KD Validated
human	WB	Acta Biochim Biophys Sin (Shanghai); Magnolol promotes the autophagy of esophageal carcinoma cells by upregulating HACE1 gene expression; Authors - Kenan Huang
human	IHC	Cancer Med; Overexpression of HACE1 in gastric cancer inhibits tumor aggressiveness by impeding cell proliferation and migration.; Authors - Ying-Ling Chen ; KO Validated
human	IHC	Transl Cancer Res; Unveiling the role of HACE1 in cervical cancer: implications for human papillomavirus infection and prognosis; Authors - Siyang Xiang
human	WB	Autophagy; HMBOX1 reverses autophagy mediated 5-fluorouracil resistance through promoting HACE1-induced ubiquitination and degradation of ATG5 in colorectal cancer; Authors - Yan Gao