HACL1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

HACL1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15519-1-AP

Synonyme

2-hydroxyphytanoyl-CoA lyase, 2-hydroxyacyl-CoA lyase 1, 2-HPCL, 2 hydroxyphytanoyl CoA lyase, 2 hydroxyacyl CoA lyase 1

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  WB analysis of HeLa using 15519-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15519-1-AP (HACL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 15519-1-AP

  WB result of HACL1 antibody (15519-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HACL1 transfected HEK-293 cells.

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  WB analysis of HEK-293 using 15519-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15519-1-AP (HACL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 15519-1-AP

  IP result of anti-HACL1 (IP:15519-1-AP, 4ug; Detection:15519-1-AP 1:300) with HeLa cells lysate 2640 ug.

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  IHC staining of rat liver using 15519-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 15519-1-AP (HACL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat liver using 15519-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 15519-1-AP (HACL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 15519-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15519-1-AP (HACL1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of rat liver using 15519-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver using 15519-1-AP (HACL1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of rat liver using 15519-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver using 15519-1-AP (HACL1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of rat kidney using 15519-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney using 15519-1-AP (HACL1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of rat kidney using 15519-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney using 15519-1-AP (HACL1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 15519-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15519-1-AP (HACL1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of rat liver using 15519-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver slide using 15519-1-AP (HACL1 Antibody) at dilution of 1:50.

• 

  IHC staining of rat liver using 15519-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver slide using 15519-1-AP (HACL1 Antibody) at dilution of 1:50.

• 

  IF Staining of HeLa using 15519-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 15519-1-AP (HACL1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: Rattenlebergewebe, humanes Lebergewebe, Rattennierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

15519-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA HACL1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HACL1 fusion protein Ag7830
• Vollständiger Name: 2-hydroxyacyl-CoA lyase 1
• Berechnetes Molekulargewicht: 64 kDa
• Beobachtetes Molekulargewicht: 64 kDa
• GenBank-Zugangsnummer: BC001627
• Gene symbol: HACL1
• Gene ID (NCBI): 26061
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.