HADH Polyklonaler Antikörper

HADH Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

18

Kat-Nr. : 19828-1-AP

Synonyme

HAD, HADH, HADH1, HADHSC, HCDH, HHF4, M/SCHAD, SCHAD

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Galerie der Validierungsdaten

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  WB analysis using 19828-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19828-1-AP (HADH antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 19828-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 19828-1-AP (HADH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 19828-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 19828-1-AP (HADH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 19828-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 19828-1-AP (HADH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 19828-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 19828-1-AP (HADH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 19828-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 19828-1-AP (HADH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 19828-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 19828-1-AP (HADH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 19828-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 19828-1-AP (HADH antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 19828-1-AP

  IP Result of anti-HADH (IP:19828-1-AP, 3ug; Detection:19828-1-AP 1:700) with HepG2 cells lysate 400ug.

• 

  IHC staining of human breast cancer using 19828-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19828-1-AP (HADH antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 19828-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 19828-1-AP (HADH antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 19828-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 19828-1-AP (HADH antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human hepatocirrhosis using 19828-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 19828-1-AP (HADH antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human hepatocirrhosis using 19828-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 19828-1-AP (HADH antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human hepatocirrhosis using 19828-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 19828-1-AP (HADH antibody) at dilution of 1:400 (under 10x lens).

• 

  IHC staining of human hepatocirrhosis using 19828-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 19828-1-AP (HADH antibody) at dilution of 1:400 (under 40x lens).

• 

  IF Staining of HepG2 using 19828-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 19828-1-AP (HADH antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, Jurkat-Zellen, Mausherzgewebe, Mauslebergewebe, Mausnierengewebe, Maus-Pankreasgewebe, Maus-Skelettmuskelgewebe, Rattenherzgewebe
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Leberkarzinomgewebe, humanes Leberzirrhosegewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 17 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

19828-1-AP bindet in WB, IP, IHC, IF, ELISA HADH und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HADH fusion protein Ag13928
• Vollständiger Name: hydroxyacyl-Coenzyme A dehydrogenase
• Berechnetes Molekulargewicht: 314 aa, 34 kDa
• Beobachtetes Molekulargewicht: 30-34 kDa
• GenBank-Zugangsnummer: BC000306
• Gene symbol: HADH
• Gene ID (NCBI): 3033
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HADH(Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial) is also named as HAD, HADHSC, SCHAD and belongs to the 3-hydroxyacyl-CoA dehydrogenase family. It catalyzes the reversible dehydrogenation of 3-hydroxyacyl-CoAs to their corresponding 3-ketoacyl-CoAs with concomitant reduction of NAD to NADH and exerts it highest activity toward 3-hydroxydecanoyl-CoA. . Human HADH encodes a deduced 314-amino acid protein composed of a 12-residue mitochondrial import signal peptide and a 302-residue mature HADH protein with a calculated molecular mass of 34.3 kD.

Publikationen

Species	Application	Title
mouse	WB	Cell Metab; Pyruvate-supported flux through medium-chain ketothiolase promotes mitochondrial lipid tolerance in cardiac and skeletal muscles; Authors - Timothy R Koves
human	IP, WB	Hepatology; Disruption of the histidine triad nucleotide-binding hint2 gene in mice affects glycemic control and mitochondrial function.; Authors - Martin Juliette J
mouse	WB	EMBO Rep; SCFJFK is functionally linked to obesity and metabolic syndrome.; Authors - Lin He
rat	WB	EBioMedicine; PPARγ maintains the metabolic heterogeneity and homeostasis of renal tubules.; Authors - Zhongshi Lyu
human	WB	Front Oncol; Multi-Omics Analysis of Fatty Acid Metabolism in Thyroid Carcinoma.; Authors - Jinghui Lu
mouse	WB	Sci Rep; Targeted Metabolomics Reveals Abnormal Hepatic Energy Metabolism by Depletion of β-Carotene Oxygenase 2 in Mice.; Authors - Lei Wu