HADHA Monoklonaler Antikörper

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HADHA Monoklonal Antikörper für WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3E9B1

Kat-Nr. : 60250-1-PBS

Synonyme

3E9B1, 78 kDa gastrin binding protein, 78 kDa gastrin-binding protein, EC:2.3.1.-, ECHA

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  WB analysis of HEK293 cells using 60250-1-Ig (same clone as 60250-1-PBS)

  WB result of HADHA specific antibody (60250-1-Ig, 1:4000) with si-control and si-HADHA transfected HEK293 cells. This data was developed using the same antibody clone with 60250-1-PBS in a different storage buffer formulation.

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  WB analysis using 60250-1-Ig (same clone as 60250-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60250-1-Ig (HADHA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60250-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 60250-1-Ig (same clone as 60250-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 60250-1-Ig (HADHA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60250-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 60250-1-Ig (same clone as 60250-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 60250-1-Ig (HADHA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60250-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 60250-1-Ig (same clone as 60250-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 60250-1-Ig (HADHA antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60250-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 60250-1-Ig (same clone as 60250-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 60250-1-Ig (HADHA antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60250-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 60250-1-Ig (same clone as 60250-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 60250-1-Ig (HADHA antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60250-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 60250-1-Ig (same clone as 60250-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde ) fixed HepG2 cells using 60250-1-Ig(HADHA antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60250-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50412-1

  Cytometric bead array standard curve of MP50412-1, HADHA Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60250-1-PBS. Detection antibody: 60250-2-PBS. Standard:Ag1211. Range: 1.563-100 ng/mL.

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  Affinity and Kinetic Characterization of 60250-1-Ig

  Biolayer interferometry (BLl) kinetic assay of 60250-1-Ig against Human HADHA was performed. The affinity constant is 17.3 nM.

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Geprüfte Anwendungen

Produktinformation

60250-1-PBS bindet in WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA HADHA und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: HADHA fusion protein Ag1211
• Vollständiger Name: hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein), alpha subunit
• Berechnetes Molekulargewicht: 83 kDa
• Beobachtetes Molekulargewicht: 79 kDa
• GenBank-Zugangsnummer: BC009235
• Gene symbol: HADHA
• Gene ID (NCBI): 3030
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HADHA(Trifunctional enzyme subunit alpha, mitochondrial) is also named as HADH,78 kDa gastrin-binding protein.It belongs to the enoyl-CoA hydratase/isomerase family in the N-terminal section and the 3-hydroxyacyl-CoA dehydrogenase family in the central section.It harbors the 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities.Defects in HADHA are a cause of trifunctional protein deficiency (TFP deficiency) and long-chain 3-hydroxyl-CoA dehydrogenase deficiency (LCHAD deficiency) and maternal acute fatty liver of pregnancy (AFLP).