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SPINT1/HAI-1 Monoklonaler Antikörper

SPINT1/HAI-1 Monoklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2H2D8

Kat-Nr. : 67031-1-PBS

Synonyme

HAI 1, HAI-1, SPINT1, 2H2D8, HAI

Filter:
  • Western Blot (WB) analysis of human placenta tissue using SPINT1/HAI-1 Monoclonal antibody (67031-1-Ig)

    WB analysis of human placenta using 67031-1-Ig (same clone as 67031-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 67031-1-Ig (HAI-1 antibody) at dilution of 1:900 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67031-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using SPINT1/HAI-1 Monoclonal antibody (67031-1-Ig)

    IF Staining of MCF-7 using 67031-1-Ig (same clone as 67031-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using HAI-1 antibody (67031-1-Ig, Clone: 2H2D8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67031-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

67031-1-PBS bindet in WB, IF/ICC, Indirect ELISA SPINT1/HAI-1 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen SPINT1/HAI-1 fusion protein Ag26733
Vollständiger Name serine peptidase inhibitor, Kunitz type 1
Berechnetes Molekulargewicht 58 kDa
Beobachtetes Molekulargewicht 58 kDa
GenBank-ZugangsnummerBC004140
Gene symbol HAI-1
Gene ID (NCBI) 6692
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Hepatocyte growth factor activator inhibitor type 1 (HAI-1) is also named as SPINT1 and Kunitz-type protease inhibitor 1. HAI-1 is critical to control matriptase proteolytic activity (PMID: 25310042). HAI-1 inhibits matriptase activity through forming complex with activated matriptase (PMID: 30370662). matriptase, HAI‐1 also binds to and inhibits a variety of serine proteases including of hepsin, plasmin, prostasin, TMPRSS13 and HAT. HAI-1 as a cell-autonomous tumor suppressor that negatively regulates the HGF pathway (PMID: 25925948). HAI-1 is inhibitor of HGFAC (PMID: 9045658). HAI-1 inhibits serine protease activity of ST14/matriptase in vitro (PMID: 28710277). HAI-1 inhibits serine protease activity of TMPRSS13, via the BPTI/Kunitz inhibitor 1 domain (PMID: 20977675). HAI-1 expression is reduced in many cancer types, which is implicated in the progression of cancer and is associated with a worse prognosis in cancer patients (PMID: 30370662).