HAP1 Polyklonaler Antikörper

HAP1 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 25133-1-PBS

Synonyme

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  WB analysis of fetal human brain using 25133-1-AP (same clone as 25133-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 25133-1-AP (HAP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25133-1-PBS in a different storage buffer formulation.

• 

  WB analysis of fetal human brain using 25133-1-AP (same clone as 25133-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 25133-1-AP (HAP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25133-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 25133-1-AP (same clone as 25133-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25133-1-AP (HAP1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 25133-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 25133-1-AP (same clone as 25133-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25133-1-AP (HAP1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 25133-1-PBS in a different storage buffer formulation.

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  IF Staining of SH-SY5Y using 25133-1-AP (same clone as 25133-1-PBS)

  Immunofluorescent analysis of SH-SY5Y cells using 25133-1-AP (HAP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25133-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

25133-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA HAP1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HAP1 fusion protein Ag17705
• Vollständiger Name: huntingtin-associated protein 1
• Berechnetes Molekulargewicht: 671 aa, 76 kDa
• Beobachtetes Molekulargewicht: 75-80 kDa
• GenBank-Zugangsnummer: BC156728
• Gene symbol: HAP1
• Gene ID (NCBI): 9001
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HAP1 (Huntingtin-associated protein 1) was originally identified as neuronal protein that specifically associates with HTT/huntingtin and the binding is enhanced by an expanded polyglutamine repeat within HTT. HTT is expressed ubiquitously, while HAP1 is expressed predominantly in the central nervous system (CNS). Both HTT and HAP1 participate in intracellular trafficking. HAP1 is involved in the vesicular transport, gene transcription regulation, membrane receptor trafficking and other functions such as calcium release and protein aggregation (PMID: 19262167).