HAPLN1 Polyklonaler Antikörper

HAPLN1 Polyklonal Antikörper für IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 26831-1-PBS

Synonyme

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  IHC staining of mouse cerebellum using 26831-1-AP (same clone as 26831-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 26831-1-AP (HAPLN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26831-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 26831-1-AP (same clone as 26831-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 26831-1-AP (HAPLN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26831-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse placenta using 26831-1-AP (same clone as 26831-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 26831-1-AP (HAPLN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26831-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse placenta using 26831-1-AP (same clone as 26831-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 26831-1-AP (HAPLN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26831-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 26831-1-AP (same clone as 26831-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using HAPLN1 antibody (26831-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 26831-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

26831-1-PBS bindet in IHC, IF/ICC, Indirect ELISA HAPLN1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HAPLN1 fusion protein Ag25221
• Vollständiger Name: hyaluronan and proteoglycan link protein 1
• Berechnetes Molekulargewicht: 40 kDa
• Beobachtetes Molekulargewicht: 40 kDa
• GenBank-Zugangsnummer: BC057808
• Gene symbol: HAPLN1
• Gene ID (NCBI): 1404
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HAPLN1 (hyaluronan and proteoglycan link protein 1), also known as CRTL1. It is expected to be located in the cytoplasm and extracellular, and the protein is mainly expressed in placenta and small intestine. The calculated molecular weight of HAPLN1 is 40 kDa. HAPLN1 binds to aggrecan on the hyaluronic chain, stabilizing the aggregates and thus increasing compression resistance and shock absorption. HAPLN1 also functions as a growth factor, up regulating aggrecan and type II collagen synthesis in cartilage. It seems to be an essential part of cartilage homeostasis and may also contribute to homeostasis in the disc tissue (PMID: 23537453).