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HARS Monoklonaler Antikörper

HARS Monoklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1B7G2

Kat-Nr. : 67662-1-PBS

Synonyme

FLJ20491, HARS, HisRS, Histidine tRNA ligase, histidyl tRNA synthetase, HRS

Filter:
  • Western Blot (WB) analysis of various lysates using HARS Monoclonal antibody (67662-1-Ig)

    WB analysis using 67662-1-Ig (same clone as 67662-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67662-1-Ig (HARS antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67662-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using HARS Monoclonal antibody (67662-1-Ig)

    IF Staining of MCF-7 using 67662-1-Ig (same clone as 67662-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using HARS antibody (67662-1-Ig, Clone: 1B7G2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67662-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

67662-1-PBS bindet in WB, IF/ICC, Indirect ELISA HARS und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen HARS fusion protein Ag9224
Vollständiger Name histidyl-tRNA synthetase
Berechnetes Molekulargewicht 509 aa, 57 kDa
Beobachtetes Molekulargewicht 50 kDa
GenBank-ZugangsnummerBC011807
Gene symbol HARS
Gene ID (NCBI) 3035
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HARS is a cytoplasmic enzyme that belongs to the class II family of aminoacyl-tRNA synthetases. The enzyme is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. HARS is located in a head-to-head orientation with HARSL on chromosome five, where the homologous genes share a bidirectional promoter. The gene product is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis.