HARS2 Polyklonaler Antikörper

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• KD/KO Validated

HARS2 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 11301-1-AP

Synonyme

HARS2, HARSL, HARSR, HisRS, Histidine tRNA ligase, Histidine tRNA ligase like, HO3

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Galerie der Validierungsdaten

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  WB analysis of MCF-7 using 11301-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11301-1-AP (HARS2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11301-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11301-1-AP (HARS2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11301-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11301-1-AP (HARS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 11301-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 11301-1-AP (HARS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 11301-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11301-1-AP (HARS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 11301-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 11301-1-AP (HARS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 11301-1-AP

  IP Result of anti-HARS2 (IP:11301-1-AP, 4ug; Detection:11301-1-AP 1:500) with HEK-293 cells lysate 3000ug.

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  IHC staining of human breast cancer using 11301-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11301-1-AP (HARS2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 11301-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11301-1-AP (HARS2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 11301-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11301-1-AP (HARS2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 11301-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11301-1-AP (HARS2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 11301-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 11301-1-AP (HARS2 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF7 cells, HEK-293-Zellen, humanes Herzgewebe, Mausnierengewebe, MCF-7-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below

Produktinformation

11301-1-AP bindet in WB, IP, IHC, ELISA HARS2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HARS2 fusion protein Ag1827
• Vollständiger Name: histidyl-tRNA synthetase 2, mitochondrial (putative)
• Berechnetes Molekulargewicht: 57 kDa
• Beobachtetes Molekulargewicht: 50-55 kDa
• GenBank-Zugangsnummer: BC014982
• Gene symbol: HARS2
• Gene ID (NCBI): 23438
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HARS2, also named as HARSL, HARSR, HO3 and HisRS, belongs to the class-II aminoacyl-tRNA synthetase family. It is an enzyme that catalyzes the esterification of histidine to its cognate tRNA as an early step in protein biosynthesis. When overexpressed in HIV-1-producing cells, HARS2 was incorporated into wild-type virions but not in ones containing the dilysine-mutated variant of matrix(MA) protein . HO3, through its recognition of MA, plays a role in the life cycle of HIV-1. (PMID: 9445076 ,7755634 ). 1-33aa of HARS2 is transit peptide and this part will be cut in some form(~52kd) of HARS2. This antibody can bind both HARSs for the close sequences.

Publikationen

Species	Application	Title
human	WB	J Biol Chem; Overexpression of mitochondrial histidyl-tRNA synthetase restores mitochondrial dysfunction caused by a deafness-associated tRNAHis mutation.; Authors - Shasha Gong
mouse	WB	Front Cell Neurosci; Disruption of Hars2 in Cochlear Hair Cells Causes Progressive Mitochondrial Dysfunction and Hearing Loss in Mice.; Authors - Pengcheng Xu ; KO Validated
mouse	WB	Cells; CLPP Depletion Causes Diplotene Arrest; Underlying Testis Mitochondrial Dysfunction Occurs with Accumulation of Perrault Proteins ERAL1, PEO1, and HARS2; Authors - Jana Key