HAS2 Rekombinanter Antikörper

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• KD/KO Validated

HAS2 Rekombinant Antikörper für WB, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240061G4

Kat-Nr. : 83204-2-PBS

Synonyme

240061G4, EC:2.4.1.212, HA synthase 2, hyaluronan synthase 2, Hyaluronate synthase 2

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  WB analysis using 83204-2-RR (same clone as 83204-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83204-2-RR (HAS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83204-2-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 83204-2-RR (same clone as 83204-2-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 83204-2-RR (HAS2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83204-2-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 83204-2-RR (same clone as 83204-2-PBS)

  WB result of HAS2 antibody (83204-2-RR; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-HAS2 transfected HEK-293 cells. This data was developed using the same antibody clone with 83204-2-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP00153-1

  Cytometric bead array standard curve of MP00153-1, HAS2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83204-2-PBS. Detection antibody: 83204-1-PBS. Standard: Ag34945. Range: 0.625-20 ng/mL.

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  Cytometric bead array standard curve of MP00153-3

  Cytometric bead array standard curve of MP00153-3, HAS2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83204-2-PBS. Detection antibody: 83204-3-PBS. Standard: Ag34945. Range: 0.625-20 ng/mL.

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  Affinity and Kinetic Characterization of 83204-2-PBS

  Biolayer interferometry (BLl) kinetic assay of 83204-2-PBS against Human HAS2 was performed. The affinity constant is 0.516 nM.

• 

  Affinity and Kinetic Characterization of 83204-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83204-2-RR against Human HAS2 were performed. The affinity constant is 1.37 nM.

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Geprüfte Anwendungen

Produktinformation

83204-2-PBS bindet in WB, Cytometric bead array, Indirect ELISA HAS2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: HAS2 fusion protein Ag34945
• Vollständiger Name: hyaluronan synthase 2
• Berechnetes Molekulargewicht: 552 aa, 64 kDa
• Beobachtetes Molekulargewicht: 63-67 kDa
• GenBank-Zugangsnummer: BC069353
• Gene symbol: HAS2
• Gene ID (NCBI): 3037
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RIT2 (Hyaluronan synthase 2) is a member of the gene family encoding the hyaluronan synthase 2, which can generate high-molecular-weight hyaluronan (HMW-HA) (PMID: 35069543). HAS2 is the most critical synthase in producing hyaluronan and is well known for its involvement in cancer growth, metabolism and metastasis (PMID: 36386154). HAS2 is involved in cellular acquired resistance to drug therapy in BrCa. HAS2 expression was decreased in the endocrine-resistant ER+BrCa cells.