HAS2 Rekombinanter Antikörper

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• KD/KO Validated

HAS2 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

240061G4

Kat-Nr. : 83204-2-RR

Synonyme

Hyaluronic acid synthase 2, Hyaluronate synthase 2, hyaluronan synthase 2, HA synthase 2, EC:2.4.1.212

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  WB analysis of HEK-293 using 83204-2-RR

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 83204-2-RR (HAS2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 83204-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83204-2-RR (HAS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 83204-2-RR

  WB result of HAS2 antibody (83204-2-RR; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-HAS2 transfected HEK-293 cells.

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  Affinity and Kinetic Characterization of 83204-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83204-2-RR against Human HAS2 were performed. The affinity constant is 1.37 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HCT 116-Zellen, HT-29-Zellen, NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83204-2-RR bindet in WB, ELISA HAS2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: HAS2 fusion protein Ag34945
• Vollständiger Name: hyaluronan synthase 2
• Berechnetes Molekulargewicht: 552 aa, 64 kDa
• Beobachtetes Molekulargewicht: 63-67 kDa
• GenBank-Zugangsnummer: BC069353
• Gene symbol: HAS2
• Gene ID (NCBI): 3037
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RIT2 (Hyaluronan synthase 2) is a member of the gene family encoding the hyaluronan synthase 2, which can generate high-molecular-weight hyaluronan (HMW-HA) (PMID: 35069543). HAS2 is the most critical synthase in producing hyaluronan and is well known for its involvement in cancer growth, metabolism and metastasis (PMID: 36386154). HAS2 is involved in cellular acquired resistance to drug therapy in BrCa. HAS2 expression was decreased in the endocrine-resistant ER+BrCa cells.