HAS3 Polyklonaler Antikörper

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• KD/KO Validated

HAS3 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, FC, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

Kat-Nr. : 15609-1-AP

Synonyme

HA synthase 3, HAS3, hyaluronan synthase 3, Hyaluronate synthase 3, Hyaluronic acid synthase 3

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  WB analysis using 15609-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15609-1-AP (HAS3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 15609-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 15609-1-AP (HAS3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 15609-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15609-1-AP (HAS3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 15609-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15609-1-AP (HAS3 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver cancer using 15609-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15609-1-AP (HAS3 Antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-3-Zellen, L02-Zellen, Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 3 publications below
• FC: See 1 publications below

Produktinformation

15609-1-AP bindet in WB, FC, IHC, ELISA HAS3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HAS3 fusion protein Ag7999
• Vollständiger Name: hyaluronan synthase 3
• Berechnetes Molekulargewicht: 63 kDa
• Beobachtetes Molekulargewicht: 63 kDa
• GenBank-Zugangsnummer: BC021853
• Gene symbol: HAS3
• Gene ID (NCBI): 3038
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HAS3(hyaluronan synthase 3) belongs to the NodC/HAS family, which are widely expressed in human tissues and appear to function in embryogenesis, wound healing and other processes associated with cellular proliferation and migration(PMID:14566823). HAS3 is involved in the synthesis of the unbranched glycosaminoglycan hyaluronan, or hyaluronic acid, which is a major constituent of the extracellular matrix. It may play a role in the progression of colon cancer and, as such, may provide novel targets for diagnostic and therapeutic interventions(PMID:14566823). It has 2 isoforms produced by alternative splicing and the protein has a glycosylation site.

Publikationen

Species	Application	Title
human	WB	Nature; A druggable copper-signalling pathway that drives inflammation; Authors - Stéphanie Solier
human,mouse	WB,IHC	J Pineal Res; Melatonin inhibits the stemness of head and neck squamous cell carcinoma by modulating HA synthesis via the FOSL1/HAS3 axis; Authors - Xinyue Luo
human	WB	Int J Mol Sci; Elevation of Hyaluronan Synthase by Magnesium Supplementation Mediated through the Activation of GSK3 and CREB in Human Keratinocyte-Derived HaCaT Cells.; Authors - Kana Marunaka
human	WB,IHC,FC	Cancers (Basel); C1q-HA Matrix Regulates the Local Synthesis of Hyaluronan in Malignant Pleural Mesothelioma by Modulating HAS3 Expression.; Authors - Romana Vidergar
		Invest Ophthalmol Vis Sci; PMID: 27092721; Authors - Anny M S Cheng
human	WB	Mol Med Rep; miR‑29a‑3p represses proliferation and metastasis of gastric cancer cells via attenuating HAS3 levels.; Authors - Feihu Bai ; KD Validated