HAT1 Monoklonaler Antikörper

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HAT1 Monoklonal Antikörper für WB, IHC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1A11F6

Kat-Nr. : 67971-1-PBS

Synonyme

1A11F6, EC:2.3.1.48, KAT1

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  WB analysis using 67971-1-Ig (same clone as 67971-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67971-1-Ig (HAT1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67971-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 67971-1-Ig (same clone as 67971-1-PBS)

  WB result of HAT1 antibody (67971-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HAT1 transfected HEK-293 cells. This data was developed using the same antibody clone with 67971-1-PBS in a different storage buffer formulation.

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  IHC staining of rat testis using 67971-1-Ig (same clone as 67971-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 67971-1-Ig (HAT1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67971-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HepG2 using 67971-1-Ig (same clone as 67971-1-PBS)

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human HAT1 (67971-1-Ig, Clone:1A11F6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67971-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67971-1-PBS bindet in WB, IHC, FC (Intra), Indirect ELISA HAT1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: HAT1 fusion protein Ag1952
• Vollständiger Name: histone acetyltransferase 1
• Berechnetes Molekulargewicht: 419 aa, 50 kDa
• Beobachtetes Molekulargewicht: 45-46 kDa
• GenBank-Zugangsnummer: BC018682
• Gene symbol: HAT1
• Gene ID (NCBI): 8520
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.