HAUS1 Polyklonaler Antikörper

HAUS1 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11094-2-PBS

Synonyme

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  WB analysis of human skeletal muscle using 11094-2-AP (same clone as 11094-2-PBS)

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11094-2-AP (HAUS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11094-2-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 11094-2-AP (same clone as 11094-2-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 11094-2-AP (HAUS1 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11094-2-PBS in a different storage buffer formulation.

• 

  WB analysis using 11094-2-AP (same clone as 11094-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11094-2-AP (HAUS1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11094-2-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 11094-2-AP (same clone as 11094-2-PBS)

  IP result of anti-HAUS1 (IP:11094-2-AP, 4ug; Detection:11094-2-AP 1:500) with HeLa cells lysate 1200ug. This data was developed using the same antibody clone with 11094-2-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 11094-2-AP (same clone as 11094-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11094-2-AP (HAUS1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11094-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 11094-2-AP (same clone as 11094-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11094-2-AP (HAUS1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11094-2-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

11094-2-PBS bindet in WB, IHC, IP, Indirect ELISA HAUS1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: HAUS1 fusion protein Ag1569
• Vollständiger Name: coiled-coil domain containing 5 (spindle associated)
• Berechnetes Molekulargewicht: 32 kDa
• Beobachtetes Molekulargewicht: 32 kDa
• GenBank-Zugangsnummer: BC014003
• Gene symbol: HAUS1
• Gene ID (NCBI): 115106
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

HAUS1 is a subunit of the human augmin complex that is involved in microtubule generation and mitotic spindle formation (PMID:27036161). In addition, HAUS1 may play a major role in centrosome integrity and completion of cytokinesis. The depletion of HAUS1 in cells decreases the G2/M cell compartment and induces apoptosis. High HAUS1 expression was associated with several tumour-infiltrating immune cells such as Th2 cells, macrophages, and activated dendritic cells (PMID:35865089). 11094-2-AP can detect the 32 kDa band, which is consistent with the calculated MW.